TranscriptionalRegulation_luxR_TU_063 – Transcriptional regulation of TU_063 by luxR

WID TranscriptionalRegulation_luxR_TU_063
Name Transcriptional regulation of TU_063 by luxR
Transcription unit TU_063 View in model
Transcripton factor MG_428_DIMER View in model
Binding site 128107129127 MG_100 MG_101
Chromosome: Mgenitalium_Chr_1, Coordinate: 128607 (nt), Length: 20 (nt), Direction: Forward, Sequence:
View in model
Fold‑change activity
1.0 (dimensionless)
View in model
Comments Canonical LuxR contains a C-terminal DNA-binding domain and an N-terminal signal binding domain. M. genitalium MG_428 contains only a DNA-binding domain. LuxR DNA-binding domain binds lux boxes with the consensus sequence NNNYRNNNNNNNNNNYRDNB [PUB_0505]. MG_103 and MG_447 were identified as possible LuxR-regulated genes using the consensus sequence and this procedure: Use the minimal luxR promoter given from the literature and find best hits in the M. genitalium genome Filter by those which have >10 bp identity with wildtype lux box Filter by those which have >4 bp identity with sites 3-5 and 16-18 Filter by those with an 8bp palindrome or greater Because MG_428 lacks the canonical N-terminal signal binding domain we have set the fold change activity of MG_428 on MG_103 and MG_447 to 1 (no effect). Searched genome for NNNYRNNNNNNNNNNYRDNB motif. Picked motif closest upstream to transcription unit. Binding site has motif NNNYRNNNNNNNNNNYRDNB.
  1. Antunes LC, Ferreira RB, Lostroh CP, Greenberg EP. A mutational analysis defines Vibrio fischeri LuxR binding sites. J Bacteriol 190, 4392-7 (2008). WholeCell: PUB_0505, PubMed: 18083819

Created 2012-10-01 15:09:29
Last updated 2012-10-01 15:18:57