Tmk – thiamine kinase

Name
WID	Tmk
Name	thiamine kinase
Cross references	EC: 2.7.1.89, BioCyc: DTMPKI-RXN, KEGG: R02094, BiGG: TMK

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + DTMP ⇔ ADP + DTDP

Catalysis
Enzyme	MG_006_DIMER [c]
Optimal pH	7.9 (dimensionless)
Optimal temperature	45.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.80
K_eq	550.954774449 ()

Kinetics
Forward kinetics	v = V_max * [DTMP] / (K_m + [DTMP]); K_m = 4.9 (nM), V_max = 0.0833 U/mg (Show evidence) Value: Rate law: VmaxDTMP/(Km+DTMP); Km = 0.0049 mM, Vmax = 0.833 umol/(minmg) (None) Measured: Yes Species: Homo sapiens References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process	Process_Metabolism

Comments
Comments	Measured Vmax=0.833 U/mg. Vmax set 10X lower to 0.0833 U/mg to fit observed mass doubling time.
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:09:02
Last updated	2012-10-01 15:17:55