Tkt1 – transketolase

Name
WID	Tkt1
Name	transketolase
Cross references	BioCyc: 1TRANSKETO-RXN, EC: 2.2.1.1, KEGG: R01641, BiGG: TKT1

Classification
Type	chemical

Reaction
Stoichiometry	[c]: R5P + X5P ⇔ S7P + T3P1

Catalysis
Enzyme	MG_066_DIMER [c]
Coenzymes	(1) TPP [c] (Show evidence) Value: TPP Measured: Yes References Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Value: TPP Measured: Yes Species: Escherichia coli K12 References Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 Value: TPP Measured: Yes Species: Escherichia coli K-12 MG1655 References UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/
Optimal pH	7.7 (dimensionless)
Optimal temperature	33.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	1.90
K_eq	0.0426031807342 ()

Kinetics
Forward kinetics	v = V_max * [R5P] * [X5P] / (K_m1 * K_m2 + K_m1 * [R5P] + K_m2 * [X5P]); K_m1 = 160 (nM), K_m2 = 1400 (nM), V_max = 50.4 U/mg (Show evidence) Value: Rate law: VmaxR5PX5P/(Km1Km2+Km1R5P+Km2X5P); Km = 0.16 mM, 1.4 mM, Vmax = 50.4 umol/(minmg) (None) Measured: Yes Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	NONOXIPENT_PWY
Process	Process_Metabolism

Comments
References	Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Keseler IM, Bonavides-Martínez C, Collado-Vides J, Gama-Castro S, Gunsalus RP, Johnson DA, Krummenacker M, Nolan LM, Paley S, Paulsen IT, Peralta-Gil M, Santos-Zavaleta A, Shearer AG, Karp PD. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res 37, D464-70 (2009). WholeCell: PUB_0006, PubMed: 18974181 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:09:02
Last updated	2012-10-01 15:17:55