ThiL – thiamine-phosphate kinase

Name
WID	ThiL
Name	thiamine-phosphate kinase
Cross references	EC: 2.7.4.16, KEGG: R00617, BiGG: TMPK

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + THMP ⇔ ADP + TPP

Catalysis
Enzyme	MG_372_DIMER [c]
Optimal pH	8.0 (dimensionless)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	0.0
K_eq	1.0 ()

Kinetics
Forward kinetics	v = V_max * [ATP] * [THMP] / (K_m1 * K_m2 + K_m1 * [ATP] + K_m2 * [THMP]); K_m1 = 1.1 (nM), K_m2 = 270 (nM), V_max = 0.000197 U/mg (Show evidence) Value: Rate law: VmaxATPTHMP/(Km1Km2+Km1ATP+Km2THMP); Km = 0.0011 mM, 0.27 mM, Vmax = 0.000197 umol/(minmg) (None) Measured: Yes Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:09:02
Last updated	2012-10-01 15:17:54