Pyk_ADP – pyruvate kinase

Name
WID	Pyk_ADP
Name	pyruvate kinase
Cross references	SABIO-RK: 12737, EC: 2.7.1.40, BioCyc: PEPDEPHOS-RXN, BiGG: PYK, KEGG: R00200

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ADP + H + PEP ⇔ ATP + PYR

Catalysis
Enzyme	MG_216_TETRAMER [c]
Optimal pH	6.8 (dimensionless)
Optimal temperature	51.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	5.30
K_eq	0.000150267274442 ()

Kinetics
Forward kinetics	v = V_max * [ADP] * [PEP] / (K_m1 * K_m2 + K_m1 * [PEP] + K_m2 * [ADP]); K_m1 = 1000 (nM), K_m2 = 170 (nM), V_max = 98.0 U/mg
Backward kinetics	(Show evidence) Value: Rate law: VmaxADP/(Km+ADP); Km = 1.0 mM, Vmax = 98 U/mg (None) Measured: Yes* Species: Lactococcus lactis (subsp. lactis) References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	GLYCOLYSIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:59
Last updated	2012-10-01 15:17:46