PrsA – phosphoribosylpyrophosphate synthetase

Name
WID	PrsA
Name	phosphoribosylpyrophosphate synthetase
Cross references	EC: 2.7.6.1, BiGG: PRPPS, BioCyc: PRPPSYN-RXN, KEGG: R01049

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + R5P ⇔ AMP + H + PRPP

Catalysis
Enzyme	MG_058_HEXAMER [c]
Optimal pH	8.0 (dimensionless)
Optimal temperature	42.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.80
K_eq	550.954774449 ()

Kinetics
Forward kinetics	v = V_max * [R5P] * [ATP] / (K_m1 * K_m2 + K_m1 * [R5P] + K_m2 * [ATP]); K_m1 = 660 (nM), K_m2 = 480 (nM), V_max = 190.0 U/mg (Show evidence) Value: Rate law: VmaxR5PATP/(Km1Km2+Km1R5P+Km2ATP); Km = 0.66 mM, 0.48 mM, Vmax = 190 umol/(minmg) (None) Measured: Yes Species: Bacillus subtilis References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:59
Last updated	2012-10-01 15:17:45