PncB – nicotinic acid mononucleotide pyrophosphorylase

Name
WID	PncB
Name	nicotinic acid mononucleotide pyrophosphorylase
Cross references	EC: 2.4.2.11, BiGG: NAMNPP, KEGG: R01724

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + (3) H + H2O + NAC + PRPP ⇒ ADP + NAMN + PI + PPI

Catalysis
Enzyme	MG_037_MONOMER [c]
Optimal pH	7.7 (dimensionless)
Optimal temperature	37.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-6.30
K_eq	35033.3689397 ()

Kinetics
Forward kinetics	v = V_max * [NAC] * [PRPP] / (K_m1 * K_m2 + K_m1 * [NAC] + K_m2 * [PRPP]); K_m1 = 22 (nM), K_m2 = 1.5 (nM), V_max = 3.0 U/mg (Show evidence) Value: Rate law: VmaxNACPRPP/(Km1Km2+Km1NAC+Km2PRPP); Km = 0.022 mM, 0.0015 mM, Vmax = 3 umol/(minmg) (None) Measured: Yes Species: Salmonella typhimurium References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	COFACTOR_PROSTHETIC_BIOSYNTHESIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:56
Last updated	2012-10-01 15:17:36