Pgk2 – phosphoglycerate kinase

Name
WID	Pgk2
Name	phosphoglycerate kinase
Cross references	SABIO-RK: 10646, EC: 2.7.2.3, KEGG: R01512

Classification
Type	chemical

Reaction
Stoichiometry	[c]: DPG + GDP ⇔ G3P + GTP

Catalysis
Enzyme	MG_300_MONOMER [c]
Optimal pH	7.4 (dimensionless)
Optimal temperature	40.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	3.00
K_eq	0.00685426773181 ()

Kinetics
Forward kinetics	v = V_max * [GDP] / (K_m + [GDP]); K_m = 300 (nM), V_max = 45.0 U/mg (Show evidence) Value: Rate law: VmaxGTP/(Km+GTP); Km = 0.25 mM, Vmax = 14 umol/(minmg) (None) Measured: Yes Species: Entamoeba histolytica References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/
Backward kinetics	v = V_max * [GTP] / (K_m + [GTP]); V_max = 14.0 U/mg (Show evidence) Value: Rate law: VmaxGDP/(Km+GDP); Km = 0.3 mM, Vmax = 45 umol/(minmg) (None) Measured: Yes Species: Entamoeba histolytica References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	GLYCOLYSIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:55
Last updated	2012-10-01 15:17:32