PfkA2 – phosphofructokinase

Name
WID	PfkA2
Name	phosphofructokinase
Cross references	EC: 2.7.1.11, SABIO-RK: 21872, KEGG: R01512

Classification
Type	chemical

Reaction
Stoichiometry	[c]: F6P + GTP ⇒ FDP + GDP + H

Catalysis
Enzyme	MG_215_TETRAMER [c]
Optimal pH	7.6 (dimensionless)
Optimal temperature	36.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.80
K_eq	550.954774449 ()

Kinetics
Forward kinetics	v = V_max * [F6P] * [GTP] / (K_m1 * [GTP] + K_m2 * [F6P] + [F6P] * [GTP]); K_m1 = 800 (nM), K_m2 = 230 (nM), V_max = 30.6 U/mg (Show evidence) Value: Rate law: VmaxF6PGTP/(Km1GTP+Km2F6P+F6PGTP); Km = 0.8 mM, 0.23 mM, Vmax = 30.6 U/mg (None) Measured: Yes* Species: Escherichia coli References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	GLYCOLYSIS
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:54
Last updated	2012-10-01 15:17:31