NOX – NADH dehydrogenase

Name
WID	NOX
Name	NADH dehydrogenase
Cross references	EC: 1.11.1.1, SABIO-RK: 15229, KEGG: R00090

Classification
Type	chemical

Reaction
Stoichiometry	[c]: H + NADH + O2 ⇒ H2O2 + NAD

Catalysis
Enzyme	MG_275_TETRAMER [m]
Coenzymes	(1) FAD [c] (Show evidence) Value: FAD Measured: Yes References Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617
Optimal pH	5.2 (dimensionless)
Optimal temperature	28.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-316.90

Kinetics
Forward kinetics	(Show evidence) Value: Rate law: VmaxNADHH2O2/(Km1H2O2+Km2NADH+NADHH2O2); Km = 6.3 uM, 0.13 mM, Vmax = 147 umol/(minmg) (None) Measured: Yes Species: Enterococcus faecalis References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_Metabolism

Comments
References	Chang A, Scheer M, Grote A, Schomburg I, Schomburg D. BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acids Res 37, D588-92 (2009). WholeCell: PUB_0570, PubMed: 18984617 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:53
Last updated	2012-10-01 15:17:26