NER_DNAExcision – DNA excision

Name
WID NER_DNAExcision View in model
Name DNA excision View in model
 
Classification
Type nucleotide_excision_repair View in model
 
Reaction
Stoichiometry [c]: ATP + H2O ⇒ ADP + H + PI View in model
 
Catalysis
Enzyme MG_244_DIMER [c] View in model
 
Energetics
Is spontaneous (pH 7.5, 25C, I = 0) False View in model
 
Kinetics
Forward kinetics Vmax = 11580.0 1/min View in model
 
Associations
Process Process_DNARepair View in model
 
Comments
Comments Oligonucleotide excision is powered by ATP hydrolysis [PUB_0531]. Several groups have studied PcrA energetics and kinetics. Wigley report that 1-23 bases are excised per ATP [PUB_0541]; Tomko et al report that PcrA catalyzes excision at the rate 193 nt/s and 1 ATP/nt [PUB_0516]. PcrA is active as a dimer [PUB_0541].
References

  1. Panyutin IG, Hsieh P. The kinetics of spontaneous DNA branch migration. Proc Natl Acad Sci U S A 91, 2021-5 (1994). WholeCell: PUB_0531, PubMed: 8134343

  2. Tomko EJ, Fischer CJ, Niedziela-Majka A, Lohman TM. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell 26, 335-47 (2007). WholeCell: PUB_0516, PubMed: 17499041

  3. Wigley DB. DNA helicases: one small step for PcrA, one giant leap for RecBC?. Curr Biol 10, R444-6 (2000). WholeCell: PUB_0541, PubMed: 10873794
 
Metadata
Created 2012-10-01 15:08:53
Last updated 2012-10-01 15:17:25