NADR2 – nicotinate-nucleotide adenylyltransferase

Name
WID	NADR2
Name	nicotinate-nucleotide adenylyltransferase
Cross references	EC: 2.7.7.18, BiGG: NNATr, KEGG: R03005

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + NH3 + NR ⇒ ADP + NMN + PI

Catalysis
Enzyme	MG_240_HEXAMER [c]
Optimal pH	8.6 (dimensionless)
Optimal temperature	80.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	0.400
K_eq	0.514590152372 ()

Kinetics
Forward kinetics	v = V_max * [NR] * [ATP] / (K_m1 * K_m2 + K_m1 * [NR] + K_m2 * [ATP]); K_m1 = 135 (nM), K_m2 = 43 (nM), V_max = 11.1 U/mg (Show evidence) Value: Rate law: VmaxNRATP/(Km1Km2+Km1NR+Km2ATP); Km = 0.135 mM, 0.043 mM, Vmax = 11.1 umol/(minmg) (None) Measured: Yes Species: Bacillus subtilis References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	NUCLEOTIDE_SALVAGE_PWY
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:52
Last updated	2012-10-01 15:17:25