MetK – methionine adenosyltransferase

Name
WID	MetK
Name	methionine adenosyltransferase
Cross references	EC: 2.5.1.6, BiGG: METAT, KEGG: R00177, BioCyc: S-ADENMETSYN-RXN

Classification
Type	chemical

Reaction
Stoichiometry	[c]: ATP + H2O + MET ⇒ AMET + PI + PPI

Catalysis
Enzyme	MG_047_TETRAMER [c]

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	-3.50
K_eq	334.743453009 ()

Kinetics
Forward kinetics	v = V_max * [MET] / (K_m + [MET]); K_m = 6 (nM), V_max = 0.039 U/mg (Show evidence) Value: Km = 6 uM, Vmax = 39 nmol/(minmg) (None) Measured: Yes* References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Process	Process_Metabolism

Comments
Comments	Observed kinetic parameters: Km=6 μM [PUB_0100], Vmax=39 nmol/(min*mg) [PUB_0100], Vmax=18.0 μM/min [PUB_0134].
References	Greene RC. Kinetic studies of the mechanism of S-adenosylmethionine synthetase from yeast. Biochemistry 8, 2255-65 (1969). WholeCell: PUB_0134, PubMed: 4307991 Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:40
Last updated	2012-10-01 15:16:51