MetF – 5,10-methylenetetrahydrofolate reductase (NADH)

Name
WID	MetF
Name	5,10-methylenetetrahydrofolate reductase (NADH)
Cross references	EC: 1.5.1.15, BioCyc: 1.5.1.15-RXN, SABIO-RK: 3556, BiGG: MTHFR2, KEGG: R01218

Classification
Type	chemical

Reaction
Stoichiometry	[c]: METTHF + NADH ⇒ MTHF + NAD

Catalysis
Enzyme	MG_228_TETRAMER [c]
Optimal pH	4.7 (dimensionless)
Optimal temperature	45.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	3.60
K_eq	0.00253019122879 ()

Kinetics
Forward kinetics	(Show evidence) Value: Rate law: VmaxMTHFNAD/(Km2+Km1NAD+Km2MTHF+MTHFNAD); Km = 1.6 mM, 0.01 mM, Vmax = 9.7 umol/(minmg) (None) Measured: Yes Species: Saccharomyces cerevisiae References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/
Backward kinetics	v = V_max * [MTHF] * [NAD] / (K_m2 + K_m1 * [NAD] + K_m2 * [MTHF] + [MTHF] * [NAD]); V_max = 9.7 U/mg

Associations
Pathways	FOLATE_METABOLISM
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:40
Last updated	2012-10-01 15:16:50