Mdh – malate dehydrogenase

Name
WID	Mdh
Name	malate dehydrogenase
Cross references	EC: 1.1.1.37, SABIO-RK: 14893, BioCyc: MALATE-DEH-RXN, BiGG: MDH, KEGG: R00342

Classification
Type	chemical

Reaction
Stoichiometry	[c]: MAL + NAD ⇔ H + NADH + OA

Catalysis
Enzyme	MG_460_TETRAMER [c]
Optimal pH	8.1 (dimensionless)
Optimal temperature	55.0 (C)

Energetics
Is spontaneous (pH 7.5, 25C, I = 0)	False
ΔG (pH 7.5, 25C, I = 0; kJ mol^‑1)	6.10
K_eq	3.97912485152e-05 ()

Kinetics
Forward kinetics	v = V_max * [MAL] / (K_m + [MAL]); K_m = 20 (nM), V_max = 11.0 U/mg (Show evidence) Value: Rate law: VmaxMAL/(Km+MAL); Km = 0.02 mM, Vmax = 11 µmol/(minmg) (None) Measured: Yes Species: Methanococcus jannaschii References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/
Backward kinetics	v = V_max * [OA] * [NADH] / (K_m1 * K_m2 + K_m1 * [NADH] + K_m2 * [OA]); V_max = 29.0 U/mg (Show evidence) Value: Rate law: VmaxOA/(Km+OA); Km = 0.25 mM, Vmax = 29 µmol/(minmg) (None) Measured: Yes Species: Methanococcus jannaschii References Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Associations
Pathways	CITRIC_ACID_CYCLE
Process	Process_Metabolism

Comments
References	Wittig U, Golebiewski M, Kania R, Krebs O, Mir S, Weidemann A, Anstein S, Saric J, Rojas I. In proceedings of the 3rd International workshop on Data Integration in the Life Sciences 2006. Lecture Notes in Bioinformatics 4075, 94-103 (2006). WholeCell: PUB_0100, URL: http://sabio.villa-bosch.de/

Metadata
Created	2012-10-01 15:08:40
Last updated	2012-10-01 15:16:50