MGrrnA16S – 16S ribosomal rRNA

Name
WID	MGrrnA16S
Name	16S ribosomal rRNA
Symbol	rrsA
Cross references	CMR: MGrrnA16S, BioCyc: MGrrnA16S

Classification
Type	rRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 170007 (nt), Length: 1519 (nt), Direction: Forward, G/C content: 45.6%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 CAATTTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCAT GCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACG GGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGA AAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTG AAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTA GTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGA AGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAG GCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGC CGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAA GAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATA AGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAG CGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGT CTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAG TCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGC GTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTA CTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTA GTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCG GTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAG AATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATG TTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTT GGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCA TGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGA GCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAA TTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGG GCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAA GTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTC GTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGG TGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCT GGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGCGCATGTCAAG GATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAA CGTGGGGGTGGATCACCTC 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1519
Transcription unit	TU_088
Empirical formula (pH 7.5)	H₁₆₃₂₆C₁₄₈₀₆N₆₄₄₃O₁₀₅₇₆P₁₅₁₉
Molecular weight (pH 7.5; Da)	500789.42

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	2920.72 (dimensionless) (Show evidence) Value: 2920.72 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	1200.0 (min) (Show evidence) Value: 1200 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	15378900
pI	3.83

Function
Complex subunit	30S ribosomal subunit [c]: MGrrnA16S + MG_070_MONOMER + MG_087_MONOMER + MG_088_MONOMER + MG_090_MONOMER + MG_092_MONOMER + MG_150_MONOMER + MG_155_MONOMER + MG_157_MONOMER + MG_160_MONOMER + MG_164_MONOMER + MG_165_MONOMER + MG_168_MONOMER + MG_175_MONOMER + MG_176_MONOMER + MG_311_MONOMER + MG_417_MONOMER + MG_424_MONOMER + MG_446_MONOMER + MG_481_MONOMER + MG_522_MONOMER ⇒ RIBOSOME_30S

Comments
Comments	Modifications were first compiled for E. coli [PUB_0105, PUB_0119, PUB_0120], and then modifications generated by homologous enzymes were mapped onto M. genitalium by sequence alignment. Modification reaction kinetics were compiled from the primary literature (see Note_RNAModification).
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Gustafsson C, Reid R, Greene PJ, Santi DV. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes. Nucleic Acids Res 24, 3756-62 (1996). WholeCell: PUB_0120, PubMed: 8871555 Huang L, Ku J, Pookanjanatavip M, Gu X, Wang D, Greene PJ, Santi DV. Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA. Biochemistry 37, 15951-7 (1998). WholeCell: PUB_0119, PubMed: 9843401 Peil L. Ribosome assembly factors in Escherichia coli. Tartu University (2009). WholeCell: PUB_0105, URL: http://dspace.utlib.ee/dspace/handle/10062/10503 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:34
Last updated	2012-10-01 15:13:57