MG_470 – CobQ/CobB/MinD/ParA nucleotide binding domain

Name
WID	MG_470
Name	CobQ/CobB/MinD/ParA nucleotide binding domain
Symbol	soj
Synonyms	parA
Protein product	MG_470_MONOMER
Cross references	GenBank: AAC72491.1, CMR: MG_470, BioCyc: MG_470, SwissProt: P47706
Homologs	B. subtilis: BSU40970 M. pneumoniae: MPN_688 S. coelicolor: SCO1772 S. oneidensis: SO_4756

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 579224 (nt), Length: 810 (nt), Direction: Reverse, G/C content: 29.9%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 ATGATCATTTCTTTTGTAAATAATAAAGGCGGCGTATTAAAAACTACAAT GGCAACTAATGTTGCAGGATCATTGGTTAAACTTTGTCCAGAAAGGCGAA AAGTCATTCTTGATCTTGACGGGCAGGGGAATGTTTCTGCATCATTTGGA CAAAACCCTGAAAGATTGAATAACACGTTAATTGACATTTTGTTGAAGGT TCCAAAATTCAGTGGTTCAAATAATTTCATTGAAATAGATGATTGCTTGC TATCTGTATATGAGGGACTTGATATTCTTCCTTGTAATTTTGAACTTAAT TTTGCTGATATAGACATATCACGTAAAAAATATAAGGCCTCAGATATAGC AGAAATAGTTAAGCAACTAGCAAAAAGATATGAATTTGTTTTATTAGATA CACCACCAAATATGGCAACTTTAGTTTCTACTGCAATGAGTTTATCAGAT GTCATAGTTATTCCCTTTGAACCAGATCAATATTCGATGCTAGGTTTGAT GAGAATAGTTGAAACAATAGATACATTTAAGGAAAAAAATACAAATCTTA AAACTATTTTAGTTCCTACAAAAGTTAACGTTCGTACTCGTCTTCATAAT GAAGTAATAGATTTAGCTAAAACAAAAGCTAAAAAAAATAATGTAGCTTT TTCTAAAAATTTTGTTTCTCTGACATCAAAATCATCAGCAGCTGTAGGTT ATGAAAAATTGCCAATATCATTAGTATCATCACCAAGTAAAAAATATCTA AATGAGTATTTAGAAATAACAAAAGAAATCCTAAATTTAGCAAATTACAA TGTCCATTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 810
Transcription unit	TU_335
Empirical formula (pH 7.5)	H₈₆₄₉C₇₈₃₈N₃₂₆₄O₅₆₂₇P₈₁₀
Molecular weight (pH 7.5; Da)	263692.46

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	3.65533 (dimensionless) (Show evidence) Value: 3.65533 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	8442300
pI	3.93

Comments
Comments	ATPases involved in chromosome partitioning [PUB_0110]; Soj is a transcriptional regulator and can repress the expression of several early stage sporulation genes [PUB_0135]; parB not present in M. genitalium; parB binds to several specific sites close to the origin of DNA replication [PUB_0135]; in the presence of Soj, the small complexes of bound Spo0J (parB) are condensed into compact foci [PUB_0135]; ATP-dependent dimerization [PUB_0335];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Doubrovinski K, Howard M. Stochastic model for Soj relocation dynamics in Bacillus subtilis. Proc Natl Acad Sci U S A 102, 9808-13 (2005). WholeCell: PUB_0135, PubMed: 15998739 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Leonard TA, Butler PJ, Löwe J. Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch. EMBO J 24, 270-82 (2005). WholeCell: PUB_0335, PubMed: 15635448 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:31
Last updated	2012-10-01 15:13:48