MG_463 – dimethyladenosine transferase

Name
WID MG_463 View in model
Name dimethyladenosine transferase View in model
Symbol ksgA
Synonyms rsmA
Protein product MG_463_MONOMER
Cross references GenBank: AAC72483.1, CMR: MG_463, BioCyc: MG_463, SwissProt: P47701
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 565127570907 MG_461 MG_462 MG_463 MG_464 MG_465 MG_467 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 567627 (nt), Length: 780 (nt), Direction: Reverse, G/C content: 28.2%, Sequence:
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751
GTGAATAGTTTTTTTCCTTCACGTAAATTAGGTCAAAATTTTACGGTTAA
TTTAAGCGTCATTAAAAGAATTTTTGCTTTTGTTAAAAATTTAAATCCAC
AAGCAATTGTTGAAATAGGTGTTGGTAAAGGAGCGTTAACAAATTATTTG
TTAAAACTCAAAATACCTTACAAGGGGATAGAAATTGATAAACGCTTAAT
TGAATATCTTCTAGTTGAAAAGATATTAACTGAAGACCAACTAGTTAAAG
GCGATATTCTCAAAAAGGACTTTAATAGTTTTTTTGAAAATTTAAGTCCA
TTATTGTGTGGTAATATCCCATATAGCATTACATCTCCAATAATCAATAA
GTTTTTAGAATCAAAGCTTCGAAGCTTTGTTTTAATGACACAAAAAGAGT
TTGCTAATCGGCTTCTGGCAAAGGTTAATTCTAGTGATTATAGTGCCTTT
GGTGCTTTTTGTCAATACTATTTGACTATTACAACAGTTTTTAAAATTGA
TAGGCATGCTTTTAAACCTAAACCTAAGGTGGATTCAACCTTAATATTAT
TAGAAAAAAATAAATCGGTTAGTTATGACTTTAAGTTTGGTCTGTTTTTA
AAGCAATGTTTCAATCAAAGAAGGAAGATGTTAATTAATAACTTAAAGCA
TTTTTTTGCAGTTGATTATTTACTTAACATTATTCAAAAGCAAAATTTAA
AGACTAGCATTAGAGCGCAAGAGTTGAGTCCTTGTCAACTCTTTAATCTT
TACCAAAACATTTGTAATGGAAAAAATTAG
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780
 View in model
Transcription unit TU_331 View in model
Empirical formula (pH 7.5) H8301C7520N3060O5461P780
Molecular weight (pH 7.5; Da) 253079.83 View in model
 
Functional genomics
Is essential
No (Show evidence)
 View in model
Relative expression
1.96428 (dimensionless) (Show evidence)
 View in model
Half life
2.2 (min) (Show evidence)
 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)		 8128400
pI 4.18
 
Comments
Comments catalyzes m62A1518 and m62A1519 modifications of 16S rRNA [PUB_0105]; may have DNA glycosylase / AP lyase activity for C mispaired with oxidized T [PUB_0105, PUB_0106]; may act as quality control element segregating mature from immature 30S ribosomes [PUB_0105];
References

  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  3. Peil L. Ribosome assembly factors in Escherichia coli. Tartu University (2009). WholeCell: PUB_0105, URL: http://dspace.utlib.ee/dspace/handle/10062/10503

  4. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

  5. Zhang-Akiyama QM, Morinaga H, Kikuchi M, Yonekura S, Sugiyama H, Yamamoto K, Yonei S. KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli. Nucleic Acids Res 37, 2116-25 (2009). WholeCell: PUB_0106, PubMed: 19223326
 
Metadata
Created 2012-10-01 15:07:30
Last updated 2012-10-01 15:13:47