MG_457 – ATP-dependent metalloprotease FtsH

Name
WID	MG_457
Name	ATP-dependent metalloprotease FtsH
Symbol	ftsH
Protein product	MG_457_MONOMER
Cross references	GenBank: AAC72477.1, CMR: MG_457, BioCyc: MG_457, SwissProt: P47695
Homologs	E. coli: b3178 B. subtilis: BSU00690 M. hyopneumoniae: mhp175 M. mobile: mhp175 M. pneumoniae: MPN_671 ... 2 more S. coelicolor: SCO3404 S. oneidensis: SO_1197

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 560496 (nt), Length: 2109 (nt), Direction: Reverse, G/C content: 34.6%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 1801 1851 1901 1951 2001 2051 2101 ATGAAAAAAAGAAATAAGGGTTTAGTAGAACAAACAACTACTGAAAAAAA TAATTTTTCACGTAAAACTGCTTGAAAAGTCTTTTGATGAGTCATCATTT TAGCTGTTGTTATTGGTGTTTTAGCTTATATTTTCAGTCCAAGAGCTGCT ACTGCAGTAGTTGAAAGCTGAAAATTAAATGGAGGTAGTAACAGCACTTT AACAGCAAAAGTAAGCGGTTTTAGTAATGAACTGACATTTAAACAAATAA ATGGTTCAACTTATGTTACTGATACCATTCTCCAAGTTTCCATTACCTTT GATGGTTTAAATAGTCCATTAACTGTTACTGCTCACAAAACTGTTAATAG TAATGGCAATGTTATCTTTAATATTGCTAACTTATCAATTAACCAAAGTA ATGGTCAGATTACCGTTAATAGTAATGGAACCATGATGAATGGTGGTTCT AGTAATAACACAAAGAGTATTGCAGGTTTTGAAACCCTTGGTACTTTCAT TGCTCCTGATACTAGAGCTAGAGATGTATTAAATGGTTTGTTTGGCTTGC TACCAATTATTATCTTTGTAGTTTTCTTTTTACTCTTTTGAAGAAGTGCT AGGGGTATATCTGCAGGGGGCAGAGAAGAAGATAATATTTTTTCTATTGG CAAAACCCAAGCTAAGTTGGCTAAGTCAACTGTGAAATTTACCAATATTG CTGGACTTCAAGAGGAAAAGCATGAGTTGCTTGAGATAGTTGATTATTTA AAAAATCCATTGAAATATGCCCAGATGGGAGCAAGATCCCCACGTGGGGT AATTTTATACGGTCCACCTGGGACAGGTAAAACATTATTAGCTAAAGCAG TAGCTGGTGAAGCTGGTGTTCCTTTCTTTCAATCAACGGGTTCTGGATTT GAAGATATGCTTGTTGGTGTTGGTGCTAAACGAGTTAGAGATCTTTTCAA TAAAGCTAAAAAGGCTGCTCCTTGTATTATTTTTATTGATGAAATTGATT CAGTTGGTTCTAAACGGGGTAGAGTTGAACTCTCTTCTTATTCTGTTGTT GAGCAAACCTTAAACCAATTGTTAGCTGAAATGGATGGATTTACAAGCAG AACAGGTGTTGTTGTAATGGCAGCTACAAATAGGTTAGATGTATTAGATG ATGCATTATTAAGACCTGGAAGATTTGATAGACATATTCAAATCAATCTC CCTGATATTAAAGAAAGGGAAGGGATTTTAAAAGTTCATGCTGAAAATAA AAATCTCTCTTCTAAGATAAGTCTTTTAGATGTTGCTAAGAGAACTCCTG GGTTTTCAGGTGCTCAATTAGAAAATGTTATCAATGAAGCTACATTGTTA GCAGTTAGAGACAACCGTACCACAATTAACATTAATGACATTGATGAAGC AATTGATAGAGTAATAGCTGGTCCTGCTAAAAAGTCACGTGTAATTAGTG ATGAAGATAGAAAACTAGTTGCTTATCATGAGGCTGGTCATGCCTTGGTT GGTTTACATGTCCACAGTAATGATGAAGTACAAAAGATTACCATTATTCC TCGTGGTCAAGCAGGGGGTTACACACTTTCAACACCTAAGAGTGGTGATC TTAACCTAAAAAGAAAATCTGATTTACTTGCAATGATAGCAACTGCTATG GGCGGTAGAGCTGCTGAAGAGGAAATCTATGGTAATTTAGAAATTACTAC TGGCGCTTCTAGCGATTTTTATAAAGCAACTAATATTGCAAGAGCAATGG TAACCCAGCTTGGGATGTCTAAATTAGGTCAAGTGCAATATGTACCAAGT CAAGGGACACTCCCTTCTAATGTAAAACTTTATTCAGAACAAACTGCTAA AGATATTGACAATGAGATTAATTTCATTATTGAAGAACAGTATAAGAAAG CAAAAACAATCATTAAGAGTAACCGTAAGGAACTAGAATTGCTTGTAGAA GCACTTTTAATTGCTGAAACTATTTTGAAAAGTGATATTGACTTCATCCA TAAAAACACTAAACTACCACCAGAAATCTTATTGCAAAAGCAAGAACAAC AAGCAAAGCAAAAACTAAATAAATCTGAAGTAAAACCAGAAAGTGAAACA AACAGTTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1800 1850 1900 1950 2000 2050 2100 2109
Transcription unit	TU_326
Empirical formula (pH 7.5)	H₂₂₅₅₀C₂₀₄₄₀N₈₅₉₅O₁₄₆₈₅P₂₁₀₉
Molecular weight (pH 7.5; Da)	688889.37

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.2 (min) (Show evidence) Value: 2.2 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	21803600
pI	3.95

Comments
Comments	ftsH is a ATP-dependent, membrane bound metalloprotease which has been implicated in several processes Degradation of ssrA-tagged polypeptides Molecular chaperone for integral membrane proteins [PUB_0014] Dislocation of misfolded membrane proteins from the membrane [PUB_0018] Cell-division [PUB_0510] FtsH is a bidirectionally processive protease [PUB_0030]. FtsH preferentially cleaves hydrophilic and basic residues [PUB_0030]. Produces fragments 10-20 aa's [PUB_0030]. Cleaves at rate 1.8 1/min [PUB_0031]. Requires 8 ATP per 18 hydrolyzed peptide bonds [PUB_0030], 8.3 ATP/cleavage [PUB_0031].
References	Eds Lund P. Molecular Chaperones in the Cell. Oxford University Press, New York (2001). WholeCell: PUB_0014, ISBN: 9780199638673 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Bruckner RC, Gunyuzlu PL, Stein RL. Coupled kinetics of ATP and peptide hydrolysis by Escherichia coli FtsH protease. Biochemistry 42, 10843-52 (2003). WholeCell: PUB_0031, PubMed: 12962509 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Ito K, Akiyama Y. Cellular functions, mechanism of action, and regulation of FtsH protease. Annu Rev Microbiol 59, 211-31 (2005). WholeCell: PUB_0030, PubMed: 15910274 ... 3 more Wehrl W, Niederweis M, Schumann W. The FtsH protein accumulates at the septum of Bacillus subtilis during cell division and sporulation. J Bacteriol 182, 3870-3 (2000). WholeCell: PUB_0510, PubMed: 10851010 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Xie K, Dalbey RE. Inserting proteins into the bacterial cytoplasmic membrane using the Sec and YidC translocases. Nat Rev Microbiol 6, 234-44 (2008). WholeCell: PUB_0018, PubMed: 18246081

Metadata
Created	2012-10-01 15:07:30
Last updated	2012-10-01 15:13:46