MG_427 – osmotically inducible peroxidase

Name
WID	MG_427
Name	osmotically inducible peroxidase
Symbol	osmC
Protein product	MG_427_MONOMER
Cross references	GenBank: AAC72448.1, CMR: MG_427, BioCyc: MG_427, SwissProt: P47666
Homologs	E. coli: b1482 M. pneumoniae: MPN_625 S. oneidensis: SO_3495

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 533271 (nt), Length: 426 (nt), Direction: Forward, G/C content: 35.4%, Sequence: 1 51 101 151 201 251 301 351 401 ATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTAT TAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAG AAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCT GCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAAC TTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTA GTGATGGTTATTTTGGCCTACGTGAACTTAACATTCACTGAGAAATTCAC TCTCCTAATGAGGAAGAAGAGATTAAAGAATTCATTGATTTTGTAAGTAA ACGTTGTCCTGCTCATAACACTTTGCATGGAACTAGCAATTTTAAGATAA ATATTAGCGTTACTTTAGTCCACTAA 50 100 150 200 250 300 350 400 426
Transcription unit	TU_305
Empirical formula (pH 7.5)	H₄₅₅₇C₄₁₃₀N₁₇₄₀O₂₉₆₈P₄₂₆
Molecular weight (pH 7.5; Da)	139249.91

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	7.5 (min) (Show evidence) Value: 7.5 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	4335800
pI	3.92

Comments
Comments	surface accesssible [PUB_0141]; may contribute to host adherence and colonization [PUB_0141]; more closely related to osmC than ohr [PUB_0143]; H2O2 and CHP can both serve as OsmC substrates [PUB_0145]; H2O2 is a relatively poor OsmC substrate [PUB_0145];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Jenkins C, Geary SJ, Gladd M, Djordjevic SP. The Mycoplasma gallisepticum OsmC-like protein MG1142 resides on the cell surface and binds heparin. Microbiology 153, 1455-63 (2007). WholeCell: PUB_0141, PubMed: 17464059 Lesniak J, Barton WA, Nikolov DB. Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC. Protein Sci 12, 2838-43 (2003). WholeCell: PUB_0145, PubMed: 14627744 Saikolappan S, Sasindran SJ, Yu HD, Baseman JB, Dhandayuthapani S. The Mycoplasma genitalium MG_454 gene product resists killing by organic hydroperoxides. J Bacteriol 191, 6675-82 (2009). WholeCell: PUB_0143, PubMed: 19717589 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:29
Last updated	2012-10-01 15:13:42