MG_423 – metallo-beta-lactamase superfamily protein

Name
WID	MG_423
Name	metallo-beta-lactamase superfamily protein
Protein product	MG_423_MONOMER
Cross references	GenBank: AAC71647.1, CMR: MG_423, BioCyc: MG_423, SwissProt: P47662
Homologs	M. pneumoniae: MPN_621

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 529532 (nt), Length: 1686 (nt), Direction: Reverse, G/C content: 29.6%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 ATGGCTAAGATTAAATTCTTTGCGCTCGGCGGTCAAGATGAACGTGGTAA AAATTGCTATGTGCTTGAAATTGACAATGATGTGTTTATCTTTAATGTTG GTAGCTTAACACCAACAACAGCAGTTTTAGGTGTTAAAAAAATTATTCCT GATTTTAGTTGAATTCAAGAAAATCAAGCAAGAGTTAAAGGTATTTTTAT AGGTAATGCCATTACTGAAAACTTAGGATCATTAGAATTTTTGTTCCATA CAGTAGGATTTTTTCCTATTTACACAAGTTCAATAGGCGCTTCTATAATT AAAAGCAAAATTAATGAAAACAAACTTAATATTGCACGTGATAAATTGGA AATTCATGAATTAAAACCACTTGAAACAATCGAAATTTCTAATCATTCTA TTACCCCTTTTAAAGTATCAAGTTCTTTACCATCATCATTTGGATTTGCT TTAAATACTGATAATGGTTACATCGTTTTTATTGATGATTTCATTGTTTT AAATGATAAGAACATTGCTTTTGAAAATCAACTAAATCAAATTATCCCTA AACTTTCTGACAATACATTGTTATTAATAACAGGAGTTGGTTTAGTTGGT AGAAACAGTGGTTTTACAACACCTAAACACAAGTCATTAGAACAGTTAAA CAGGATCATAACTCCAGCCAAAGGCAGAATATTTGTAGCTTGTTATGATT CAAATGCTTACAGTGTTATGACTCTTGCCCAGATTGCAAGGATGCAAAAT CGTCCTTTTATTATCTATTCACAATCATTTGTGCATCTCTTTAACACAAT TGTGCGTCAAAAATTGTTTAATAACACGCATTTAAATACCATTTCTATTG AGGAGATTAACAATTCAACCAACAGTATTGTTGTTCTCACCTCACCACCT GATAAGTTGTATGCTAAATTATTCAAAATTGGAATGAACGAAGATGAAAG AATTCGTTATCGTAAGAGTGATACTTTTATCTTTATGACTCCTAAAGTTG CTGGCTATGAAGAGATAGAAGCACAAATCCTTGATGATATTGCACGTAAT GAAGTTAGTTATTACAACTTAGGTAGAGAAATCCTTTCTATCCAAGCAAG TGATGAAGATATGAAGTTTTTAGTTAGTTCTTTAAAACCAAAATATATTA TTCCAACTGGTGGACTTTATAGGGATTTTATAAACTTCACAATGGTTTTA AAACAAGCTGGCGCTGAGCAAAATCAAATCTTAATTCTTTTTAACGGTGA AGTTTTAACTATTGAAAACAAAAAACTAGATAGCAAAAAAAATGAATTGA AATTAAATCCTAAGTGTGTTGATTCTGCTGGATTACAAGAAATTGGAGCT AGCATTATGTTTGAACGTGATCAAATGTCAGAATCTGGAGTTGTGATCAT AATCATTTATTTTGATCAAAAAAAGAGTGAATTCTTAAATGAGATAACTT ATTCTTTTTTAGGAGTATCTTTAGATGTACCAGAAAAAGATAAACTAAAA ACAAAAATGGAAGAATTAATTAAAAAACAAATTAATGATATTAAGGATTT CACCACGATTAAAAAACGAATAGGTAAAGAAATTTCAAAAGAATTGAAGG TTTCTATTAAACGAGCAGTAATGAATTTATTCACCAAGATGACATCAAAA GCACCCTTAATTCTTTCAACTATTATTTCTATTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1686
Transcription unit	TU_301
Empirical formula (pH 7.5)	H₁₇₉₈₁C₁₆₂₉₄N₆₇₃₂O₁₁₇₄₈P₁₆₈₆
Molecular weight (pH 7.5; Da)	548301.28

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	17553500
pI	4.01

Comments
Comments	beta-CASP family [PUB_0163]; lacks part of all the conserved amino acids that should be involved in the enzymatic function [PUB_0163];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Callebaut I, Moshous D, Mornon JP, de Villartay JP. Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family. Nucleic Acids Res 30, 3592-601 (2002). WholeCell: PUB_0163, PubMed: 12177301 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:28
Last updated	2012-10-01 15:13:41