MG_414

Name
WID	MG_414
Protein product	MG_414_MONOMER
Cross references	GenBank: AAC71641.1, CMR: MG_414, BioCyc: MG_414, SwissProt: P47653
Homologs	M. pneumoniae: MPN_612

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 516245 (nt), Length: 3111 (nt), Direction: Reverse, G/C content: 25.7%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 1801 1851 1901 1951 2001 2051 2101 2151 2201 2251 2301 2351 2401 2451 2501 2551 2601 2651 2701 2751 2801 2851 2901 2951 3001 3051 3101 ATGCGTTCATATCTTTTTATTCCTTTATTAGTTTCTTTTCTTTTTCCCGT TGCTTTAGCTAATGCTTCACTTGAAAAAAACTTGGTAGATACTAATCTTT ATCTCTCTCCAAACACAATTCAAAACAGAAGCTATACAGCTGGATTTAAT TTAAAAAACGATTATTTTCGAAAAAGCTTTAATTTTGATAATGAAGTAAT TCAAAGACCTAATCAAAATAATTATTTTGCAAAAACTTATTCGAATGAAT GAAGTGAATTAGTTAATAAATATAGTGATAATTACAAACAAATTATTAGA GATAATAACTTTCACTGATACAACAATATTTATTATCTAAAGAACATAAA GTTAAAAATTGAGAGTAGTTATATTGCACATGCATTTTCATCTACATTTA GATACCCTGGAATTAGAAACTCATGGTCATCTGATATCCATAGTGAGCAG AGATTTTATTTTCATAGTCAAGCAATTTCTAATTTTTTTCCAAGAGATGG TCATACTAATGAAAAATGAACCGAAGTTCCTTCTAGGAATATAGAAATAA CTGGTACAAATCCAGATGGTTCTTGAAACAAAAGTTGATATAAACCTGAT AATCCTTATTTTTGAGACCTTACAAAGCCCAGTCATAAAAAAGCATATTT TATTGAAGCTAAGTTTGTTCACATGTGAGGATCGACTTTAAGAATGATTA AACCAACCCCAGAAGATGTTATTAGAAATATTAAATGATTAAGAAAAGGT AATAATGGTAATTATGTAATTGATTTTAATGCACCTATGTGATATTATCT TGGCCAAGAATCAAGAGGATTTTTTGCTGACTGAAAAAATAATATTTTTG TAGCTCCAGATTTCTGATGAGGTTTAGCAATGACACCAATTAGAGCTTAT CGTTTCTGATGAGATTTAGAACTAGTGAAAGTTCCTCCAGTTTTTGAACA GAATAACGCTCAGTGAAACGAACCTTTTCCTGGTAGAAGCAGTTGATTTT TTTCTATTAACAGCAACAAACAAGTTGATGCTCATTGGATGAGTATAAAT GAGTTTAAAGAGAAAATGAAAAAGGATAGAAAAATCATTGAAGCATTTAC TAATAATCGTGAAATAACTCAAACGATATTAAGACTAGGTAATGGATTAG TAGTTAGACCAGAAACTGAAGATAGTAGACGTGAAGCATTCAACAGTAAA TGGGATATTTTTAAACATACACCTATATTACCTGAACAAGAAGTTGTTCA TCATCAAATAAGTTTTTATCTAAGGCAAAATAATCCTGATGAAGTTTTAC CAATTAGTTTCAGTAATCTTTCAAAGCTTTGAATTTCACAGTTGGAATTT GATATTAATTCGCTTGTTAGTCAAACTGAAAAAACTCTAAATAAAGAAAC AATAGGTACAAAAATTAAACCAACTATTAAATTTAAAGATAAATTCATAA ATGCAATTAAAGAAGTTAGTTTAATTAATCAAAGAATTGATGAATCAATT GATAAAAATGAAGCGTTAAAAAGCTTTAATATTTCAACCAATAATCAAAG TAATTTTTATCCAAATTTAGATTATTTATACAACCTATTGCAAATGTCAC CAAACAATAAAGAAGAACTATTCTTTATTAGAAATTTACCAAGAATGGTC AAAACCATTTTTGATAGATCAACATTAACTGTTAAAGTAAAAATTGGTAA TTCTGTAAATGAAATAACATTGTTAAGAAATAACAAGAATTATTTTGATT TAAGTTCAATTGAGGAGTTTTTAACAGAAAAACAGAAAGCAGATAATGAA ATGAATATTGAATTTTTAGCACTTAATTTTTTTGTAGATGGATATGAATC AGAAAATATTAGTAACTATAGTGTTGAACCACTGTTTGACAGTATTAAAA AACTTTCAACTATCAAAAGAACTAAGGATGGTTTTGAATACAAATTTAAA TATAGAAAAGATTTTAATGAACAGCGTTGAATTGCTAAAGATTTTCGTAT TCCACTAAATAAAAATGTTCAAAACTTTAATGTTGCTGAACTTAAAAAAC GTAATGAAAAGTTTAATGATTATGAAAAAAGACAAATGGCTTTCATTATC AAAAATAGTTTTAAATCTAAGGATAAGAATATTGATTTAGATATCAACAG TAACTCTTTAACATTTGTGGATAACCCTAAAAAATATTTCAAACATCTAG AGCCTACTAATGAAAAGAAAGGTATATTTTATGTTTTAGCAGAAATAAAT AATAGCAATGAACTATTCAAATATGGTTCTGAAAGTGATTCTGAAATTAT TAAAGACAAAATGTATTTTCTATCACAAAATCAAAAAAACCCTAAATTAA GAACATATTTATTTAAATTTCATACTAACAAACTTTTTGTTGATAAAAAT GACCTGGGATTTAAGTTAGAAAAAGACAAGGTATTTTTATCAGTTGATAA CTTAAAAATTGCTGAATTAGATTTAAAGTCATCTTCATTTAAATTTCTAG AGGATTATCAACTTGATTTTCATGAACCTTTTAGTTTAAATGACGAACAA TTACTAGTTGATAAACTCAATATTACTCTAAGTGAAAAAAGATTGCAAAC AACTAAAAACGTCCGCTTTAACCTAAAGAATAAATTTATTAACATCCATT TAGTAGAAAATAAAAATCAATTTAATTTAGTTTTTGATGTCGATGTTAGA TCAAAAAAATTATTTATTAAAGGTGTTAATAATGATAACCAAGTATTTAG TATTAGCTATGATTTAAAAATAACAAATAATCAAACACTTTTAATTGTAG ATGCCAATGGTTTTGATAATTCTATTTGATTTGATATTACTAGTGAAAAT CAAACACAACTTTTCAAAGCATTATCATTTTATCTAAAACAAAATAACCT GCAATTCAAGAGAGTTCCAGATTTTAATCTTAAATCTCAAGATAAATCCT ATGAAGTTGATAAGTTAGAAAAAAATGAAATTAAAAAACAAGATACAAAT GTTGAGTTAATATTATGAGTCATTTTTGGAATTATCATTAGCTTAATGAT AAGTTCTGCTGTTTTGTTTTTAAAATGAAGAAAAAAGAAAATTGTTAAGA AAAGAAATTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1800 1850 1900 1950 2000 2050 2100 2150 2200 2250 2300 2350 2400 2450 2500 2550 2600 2650 2700 2750 2800 2850 2900 2950 3000 3050 3100 3111
Transcription unit	TU_297
Empirical formula (pH 7.5)	H₃₃₁₇₉C₃₀₀₆₇N₁₂₄₂₆O₂₁₅₅₂P₃₁₁₁
Molecular weight (pH 7.5; Da)	1009792.65

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	32954300
pI	3.92

Comments
Comments	Gene disruption mutants in MG414 have 16% faster doubling times than wild type M. genitalium [PUB_0182, PUB_0193];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Peterson JD, Umayam LA, Dickinson T, Hickey EK, White O. The Comprehensive Microbial Resource. Nucleic Acids Res 29, 123-5 (2001). WholeCell: PUB_0182, PubMed: 11125067, URL: http://cmr.jcvi.org/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:28
Last updated	2012-10-01 15:13:40