MG_383 – NH(3)-dependent NAD+ synthetase, putative

Name
WID	MG_383
Name	NH(3)-dependent NAD+ synthetase, putative
Symbol	nadE
Protein product	MG_383_MONOMER
Cross references	GenBank: AAC71610.1, CMR: MG_383, BioCyc: MG_383, SwissProt: P47623
Homologs	E. coli: b1740 B. subtilis: BSU03130 M. hyopneumoniae: mhp471 M. mobile: mhp471 M. pneumoniae: MPN_562 ... 2 more S. coelicolor: SCO0506 S. oneidensis: SO_2021

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 481329 (nt), Length: 747 (nt), Direction: Reverse, G/C content: 29.9%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 ATGACTAATTTAATTAAATATTTAAAAGAACTCCAAAACTGGCTGTTTGA TTATGTAAAAAAATCTAAAGCTAAAGGTGTTATTTTTGGCTTATCTGGAG GAATTGATTCAGCAGTTGTTGCTGCTATTGCTAAAGAAACTTTTGGTTTT GAAAACCATTTAGCTTTAATAATGCATATTAATAATTCAAAACTTGATTT TCAAGCAACTAGTGAACTTGTTAAAAAAATGCAATTTAATAGTATTAACA TTGAACTGGAAGAGAGTTTCAATCTGTTAGTAAAAACCCTTGGAATAGAT CCAAAAAAAGATTTTTTAACAGCTGGTAACATTAAAGCACGTTTACGGAT GATAACTTTATATGCTTATGCTCAAAAACACAACTTCTTAGTTTTAGGTA CTGGTAATTTTGTAGAGTATACACTTGGTTATTTCACAAAATGAGGAGAT GGAGCTTGTGATATTGCTCCTTTAGCATGGCTTTTAAAAGAGGACGTTTA CAAATTAGCTAAGCATTTTAATATTCCTGAAATTGTAATCACAAGAGCGC CAACTGCTAGTCTTTTTGAAGGGCAAACTGATGAGACAGAGATGGGCATT ACTTATAAGGAACTTGATCAATATTTAAAAGGTGATTTAATACTTAGTTC AGAAAAGCAAAAAATTGTTTTAGATTTGAAAGCAAAAGCAGAGCATAAAC ATAATTCACCTTTGAAATTTAAACATCTCTATAATTTCCAGAACTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747
Transcription unit	TU_278
Empirical formula (pH 7.5)	H₇₉₆₉C₇₂₂₁N₂₉₈₈O₅₂₀₄P₇₄₇
Molecular weight (pH 7.5; Da)	243011.55

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.5 (min) (Show evidence) Value: 5.5 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	7764600
pI	4.02

Comments
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:27
Last updated	2012-10-01 15:13:35