MG_376

Name
WID	MG_376
Protein product	MG_376_MONOMER
Cross references	GenBank: AAC71603.1, CMR: MG_376, BioCyc: MG_376, SwissProt: P47616
Homologs	M. pneumoniae: MPN_554

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 474578 (nt), Length: 315 (nt), Direction: Reverse, G/C content: 29.8%, Sequence: 1 51 101 151 201 251 301 ATGTTAAATTGCGTTTTTTTGGAAGGTGAAATAGAAAGCACAAAGTGAAG CCACAAAAAGACAGGATTTTTAGTAACTATTAAGCAGAAACGTTTGTTTG GTGAACGAAGTTTTACTGATTTTTTTGTTTTTTATGCAAATGGTCAATTA GCATTTGAACTTGAAGCATATACTCAAAAATTTAAAACAATTAGCATCGA AGGTATTCTGCGCACTTACCTAGAAAAACGCAGTGGAATTTGAAAAACAA CAATCGAAGTTGTCAAAATTATGAAACCAAATTCAAAAGTAATGATTGAT TATCAAGAATCTTAA 50 100 150 200 250 300 315
Transcription unit	TU_272
Empirical formula (pH 7.5)	H₃₃₆₈C₃₀₅₂N₁₂₈₁O₂₁₈₁P₃₁₅
Molecular weight (pH 7.5; Da)	102645.28

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	3282700
pI	3.87

Comments
Comments	OB protein fold (oligonucleotide/oligosaccharide/oligopeptide binding fold) family[PUB_0191]; most similar in structure to the Escherichia coli PriB [PUB_0191]; bind and stabilize transiently formed single-stranded DNA [PUB_0191];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Das D, Hyun H, Lou Y, Yokota H, Kim R, Kim SH. Crystal structure of a novel single-stranded DNA binding protein from Mycoplasma pneumoniae. Proteins 67, 776-82 (2007). WholeCell: PUB_0191, PubMed: 17348019 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:27
Last updated	2012-10-01 15:13:34