MG_367 – ribonuclease III

Name
WID	MG_367
Name	ribonuclease III
Symbol	rnc
Protein product	MG_367_MONOMER
Cross references	GenBank: AAC71594.1, CMR: MG_367, BioCyc: MG_367, SwissProt: P47607
Homologs	E. coli: b2567 B. subtilis: BSU15930 M. hyopneumoniae: mhp412 M. mobile: mhp412 M. pneumoniae: MPN_545 ... 2 more S. coelicolor: SCO5572 S. oneidensis: SO_1348

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 464646 (nt), Length: 789 (nt), Direction: Reverse, G/C content: 27.8%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 ATGAAGAATAAAGTTTTGAAACTAAAAAATAATAAGATTTTTGATAAAAA ACTAGCAACTTTTTTAAAGAATTTAGATATTTTTCCTAATAATTGAGAAT TTTTTGAAAAAGCTTTTATTCACGCTTCTTACATCAATGAACATGAAGAT GTTAGTGAAAGTTACGATCGCTTAGAGTTTTTAGGTGATGCTTTAATTGA CTTTGTTGTTGCTAAAAAACTATTTGAACTTTATCCTAAATATAACGAAG GTCTTTTAACAAGAACTAAGATTGAAATTGTTAAGGGTGAAAATCTTAAT CGTATTGGTATGGAGCTAAAATTAGGTGATTTTGTTAAGTTAAGCAATGG TGCTGAACTAACTGAAAACACTGTTGGTGATGTACTTGAAGCTTTGGTTG GCGCCATTTATGAAGATATGGGGATGAAAAAAGCAACTGAATTTGTTGAA AAATATATTTTTGAAAGAACTTTTTCTGAGATTTTAAAATACGATTTCTT TTCACTTTTTCAAGAGCAAAAATTACCTGAACCAAGAGTTAGGGTAAGCT TAACTTCAAATAATTTGGTACTTAGTATAATTGAACTTGATGGTGATATT ATTTGATCACAAGCTATCCCAAATAACAAAAATTACGATGATAAAAGTGT TTTAGAGCACAACGCAATGGCTTCTTTTACAAGTTTTTTAAAAAGTAGTA AAGGAAGCCATTTTTTTAGTGATTTAAAAGAGAAGATAGAAAATCAAAAG ATGTGTAAGAAACTAGCTATTAAACCTAAAAAAAATTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 789
Transcription unit	TU_268
Empirical formula (pH 7.5)	H₈₄₀₉C₇₆₁₉N₃₁₃₂O₅₄₉₆P₇₈₉
Molecular weight (pH 7.5; Da)	256224.97

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.0 (min) (Show evidence) Value: 4 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	8292700
pI	4.07

Comments
Comments	Double-stranded specific endonuclease [PUB_0038, PUB_0039]. Matures rRNA and scRNA [PUB_0039]. Specifically, ribonuclease III cleaves precursor 30S rRNA to 5S, 16S, and 23S precursors [PUB_0038], which are further processed by ribonuclease J and rgsA [PUB_0039]. Ribonuclese II also cleaves scRNA precursor at two sites [PUB_0042]. Ribonuclease II may also cleave the 3' of tRNA precursors, and may initiate the degradation of mRNAs by cleaving 5' UTR [PUB_0039]. Kinetic rate in E. coli 7.7 1/s [PUB_0691].
References	Amarasinghe AK, Calin-Jageman I, Harmouch A, Sun W, Nicholson AW. Escherichia coli ribonuclease III: affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage. Methods Enzymol 342, 143-58 (2001). WholeCell: PUB_0691, PubMed: 11586889 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Deutscher MP. Maturation and degradation of ribosomal RNA in bacteria. Prog Mol Biol Transl Sci 85, 369-91 (2009). WholeCell: PUB_0038, PubMed: 19215777 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Nicholson AW. Function, mechanism and regulation of bacterial ribonucleases. FEMS Microbiol Rev 23, 371-90 (1999). WholeCell: PUB_0039, PubMed: 10371039 ... 2 more Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Yao S, Blaustein JB, Bechhofer DH. Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site. Nucleic Acids Res 35, 4464-73 (2007). WholeCell: PUB_0042, PubMed: 17576666

Metadata
Created	2012-10-01 15:07:26
Last updated	2012-10-01 15:13:33