MG_360 – ImpB/MucB/SamB family protein

Name
WID	MG_360
Name	ImpB/MucB/SamB family protein
Symbol	dinB
Synonyms	umuC
Protein product	MG_360_MONOMER
Cross references	GenBank: AAC71585.1, CMR: MG_360, BioCyc: MG_360, SwissProt: Q49426
Homologs	E. coli: b0231 B. subtilis: BSU23870 M. pneumoniae: MPN_537 S. coelicolor: SCO1380 S. oneidensis: SO_1114

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 458260 (nt), Length: 1236 (nt), Direction: Reverse, G/C content: 25.4%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 ATGATTAACACCTTTACATATTTTGAACCAGAATATTTAATTGATAAAAA TTTAATTTTTCTTTATTTTGATTTTGATGCTTTTTTTGCATCTGTTGAAG AATTAGAAAATCCAGAATTAGTTAATCAACCCTTAATAGTTGGTAATCGC TTTTCAAGAAGTGTAGTTTCAACTTGTAACTATGTTGCTCGTAGTTATGG AATTAGATCTGGAATGTCTATTTTAAAAGCATTAGAACTATGTCCAAATG CTATTTTTGCACATTCTAATTTTCGTAATTACAGAAAACATTCTAAACGT ATTTTTTCAGTTATTGAAAGCACTTTTTCATTAAAAATTGATGTTCTTTC TGTTGATGAAGGGGTTGCTTGTTTTCAAAACATTTCATTTAAAAAGGCAT TTTTAATAGCGAAAAAAATAAAAAATTTTGTTTTTCAAAACTTGAGAATC AAAATTTCAATTGGCATCTCAGATCATTTTTTAATTGCTAAGATTTTTTC AAATCAAGCAAAACCATTTGGAATTAAAAGTTGTTCAGTTAAGGATATTA AAAAAAAACTTTGACCACTACCAATTACTGAAATTCCAGGGATAGGAGAA AAACATATTGATTTAGTTTTTAAAAATAATTTTTACAAAATAAATGATCT AGCAGTTTGTGAAGATGCTTCGCTTTTAAAAAAAGTATTTGGTAATTTTT GAGAATCTTTAAAAGCAGTTTCACTAGGCAAATGATATACAGATAATAAC AATCAGGTTAAGTCTCGCTCTTTTGCAGTCAGTGAAACGTTAGAAGATTT AAATTATTCAAACAATCAGTTAAATAAAAAGTTAACGCAAATATTTGATC AGCTCTTTATAAGATTACAACTCTCTTCACAAGTGTGTAAAGGGATAGTT GTGCAATTGAAAAGCAATGATTTTATTGTTAATTCTCATTCAAATAAAAT GAAAAAATATTCAAACGATTATAGAAAACTACTGTCAATAACAAAACGAT TATTCAACAGACTATTAATTAATACTGAAAAGAACGTTCGATTAATAGGT ATTAGTTTTTTTGATTTAAAAAAGATAGATACAGATGAAGGACAGAAAAA ATCTTTATTTTATCAATTCATACCAAAATCAATTAGCAAATTAAGCGAAG AATCTTCACTTGATAAATTAATTTTTGATATTAATGAAAGTTTTGGTTTT GAAATTATTAAAAGAGCTAATAAGCTTAAAAGTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1236
Transcription unit	TU_262
Empirical formula (pH 7.5)	H₁₃₁₄₈C₁₁₉₁₁N₄₈₃₃O₈₆₂₉P₁₂₃₆
Molecular weight (pH 7.5; Da)	400348.14

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.6 (min) (Show evidence) Value: 5.6 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	12983300
pI	4.13

Comments
Comments	low fidelity DNA polymerase [PUB_0136]; translesion DNA synthesis [PUB_0136]; activated by umuD, recA, SSB [PUB_0137]; polymerizes 1 base per binding event [PUB_0138]; mismatch incorporation rate 100-1000 X slower than correct match incorporation rate [PUB_0139]; allows cells to survive in the presence of relatively high levels of replication-inhibiting DNA damage, but at the cost of an increased mutation rate [PUB_0140];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Fiala KA, Suo Z. Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Biochemistry 43, 2106-15 (2004). WholeCell: PUB_0139, PubMed: 14967050 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Ohmori H, Friedberg EC, Fuchs RP, Goodman MF, Hanaoka F, Hinkle D, Kunkel TA, Lawrence CW, Livneh Z, Nohmi T, Prakash L, Prakash S, Todo T, Walker GC, Wang Z, Woodgate R. The Y-family of DNA polymerases. Mol Cell 8, 7-8 (2001). WholeCell: PUB_0136, PubMed: 11515498 Reuven NB, Arad G, Maor-Shoshani A, Livneh Z. The mutagenesis protein UmuC is a DNA polymerase activated by UmuD', RecA, and SSB and is specialized for translesion replication. J Biol Chem 274, 31763-6 (1999). WholeCell: PUB_0137, PubMed: 10542196 ... 3 more Tang M, Shen X, Frank EG, O'Donnell M, Woodgate R, Goodman MF. UmuD'(2)C is an error-prone DNA polymerase, Escherichia coli pol V. Proc Natl Acad Sci U S A 96, 8919-24 (1999). WholeCell: PUB_0140, PubMed: 10430871 Wagner J, Gruz P, Kim SR, Yamada M, Matsui K, Fuchs RP, Nohmi T. The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis. Mol Cell 4, 281-6 (1999). WholeCell: PUB_0138, PubMed: 10488344 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:26
Last updated	2012-10-01 15:13:32