MG_358_359_10MER – Holliday junction DNA helicase
Name | ||
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WID | MG_358_359_10MER |
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Name | Holliday junction DNA helicase |
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Cross references | PDB: 1HJP | |
Structure | ||
Biosynthesis | [c]: (4) MG_358_MONOMER + (6) MG_359_MONOMER ⇒ MG_358_359_10MER |
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No. subunits | 10 | |
DNA footprint | Length: 52 (nt), Binding: dsDNA, Region: dsDNA |
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Empirical formula (pH 7.5) | H22444C13808N3634O3938S84 | |
Molecular weight (pH 7.5; Da) | 305064.50 |
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Extinction coefficient (260 nm, 25C, pH 7.0) |
-168820.00 | |
Half life (OD (600 nm) = 0.3, M9 media, 36C; min) |
1200.00 |
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Synthesis | ||
Formation process | Process_MacromolecularComplexation |
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Localization | c | |
Function | ||
Enzyme | DNA recombination Holliday junction migration [c]: ATP + H2O ⇒ ADP + H + PI |
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Reaction participant |
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Complex subunit | Holliday junction DNA helicase [c]: (4) MG_358_MONOMER + (6) MG_359_MONOMER ⇒ MG_358_359_10MER |
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Parameters | ||
Parameters | Parameter_0053: HR_RuvAB_JunctionMigrationHop = 2.2 b |
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Comments | ||
Comments | RuvAB is responsible for Holliday junction migration in an ATP-dependent manner [PUB_0532]. RuvAB is composed of 1-2 RuvA tetramers and 1-2 RuvB hexameric rings [PUB_0532]. RuvA tetramers unfold the junction in a square planar conformation, energetically favorable to branch-point migration [PUB_0532]. RuvB possesses DNA-dependent ATPase activity, but this activity is extremely weak (kcat < 1/min) [PUB_0536]. RuvA stimulates RuvB ATPase activity 10X [PUB_0536]. 1 ATP binding site per RuvB monomer, but 2 ATPs bind per RuvB hexamer [PUB_0537]. Several groups have examined the kinetics of RuvAB-directed strand migration: Dawid observed 43 bp/sec under zero load [PUB_0532], Tsaneva et al observed 10-20 bp/sec in bulk [PUB_0533], Dennis et observed 50 bp/s [PUB_0534], Grigoriev and Hsieh observed 10 bp/s [PUB_0535], and Karymov et al observed 10 steps/s [PUB_0538]. Spontaneous junction migration occurs at rate 1/300 ms in presence of Mg2+, and 1/12 μs in presence of Na+ [PUB_0531]. Junction migration can be slowed by DNA mismatches [PUB_0531]. In presence of RecA migration occurs at 3 bp/s [PUB_0531]. Migration occurs in discrete hops of 1-5 bp with a mean of 2.2 bp [PUB_0538]. RuvAB is highly processive, >4431 bp [PUB_0532, PUB_0534]. Dennis et al describe a mathematical model of RuvAB-directed strand migration [PUB_0534]. DNA-binding RuvAB junction migration hop is 2.2 bp [PUB_0538]. RuvA footprint extends 13 nt on xsDNA side of crossover point [PUB_0716]. RuvAB footprint spans 50-54 nt along all four strands [PUB_0716]. Composition [PUB_0083]. | |
References |
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Metadata | ||
Created | 2012-10-01 15:07:41 | |
Last updated | 2012-10-01 15:14:14 |