MG_358_359_10MER – Holliday junction DNA helicase

WID MG_358_359_10MER View in model
Name Holliday junction DNA helicase View in model
Cross references PDB: 1HJP
Biosynthesis [c]: (4) MG_358_MONOMER + (6) MG_359_MONOMERMG_358_359_10MER View in model
No. subunits 10
DNA footprint Length: 52 (nt), Binding: dsDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H22444C13808N3634O3938S84
Molecular weight (pH 7.5; Da) 305064.50 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
Formation process Process_MacromolecularComplexation View in model
Localization c
Enzyme DNA recombination Holliday junction migration
[c]: ATP + H2OADP + H + PI
View in model
Reaction participant View in model
Complex subunit Holliday junction DNA helicase
[c]: (4) MG_358_MONOMER + (6) MG_359_MONOMERMG_358_359_10MER
View in model
Parameters Parameter_0053: HR_RuvAB_JunctionMigrationHop = 2.2 b View in model
Comments RuvAB is responsible for Holliday junction migration in an ATP-dependent manner [PUB_0532]. RuvAB is composed of 1-2 RuvA tetramers and 1-2 RuvB hexameric rings [PUB_0532]. RuvA tetramers unfold the junction in a square planar conformation, energetically favorable to branch-point migration [PUB_0532]. RuvB possesses DNA-dependent ATPase activity, but this activity is extremely weak (kcat < 1/min) [PUB_0536]. RuvA stimulates RuvB ATPase activity 10X [PUB_0536]. 1 ATP binding site per RuvB monomer, but 2 ATPs bind per RuvB hexamer [PUB_0537]. Several groups have examined the kinetics of RuvAB-directed strand migration: Dawid observed 43 bp/sec under zero load [PUB_0532], Tsaneva et al observed 10-20 bp/sec in bulk [PUB_0533], Dennis et observed 50 bp/s [PUB_0534], Grigoriev and Hsieh observed 10 bp/s [PUB_0535], and Karymov et al observed 10 steps/s [PUB_0538]. Spontaneous junction migration occurs at rate 1/300 ms in presence of Mg2+, and 1/12 μs in presence of Na+ [PUB_0531]. Junction migration can be slowed by DNA mismatches [PUB_0531]. In presence of RecA migration occurs at 3 bp/s [PUB_0531]. Migration occurs in discrete hops of 1-5 bp with a mean of 2.2 bp [PUB_0538]. RuvAB is highly processive, >4431 bp [PUB_0532, PUB_0534]. Dennis et al describe a mathematical model of RuvAB-directed strand migration [PUB_0534]. DNA-binding RuvAB junction migration hop is 2.2 bp [PUB_0538]. RuvA footprint extends 13 nt on xsDNA side of crossover point [PUB_0716]. RuvAB footprint spans 50-54 nt along all four strands [PUB_0716]. Composition [PUB_0083].
  1. Dawid A, Croquette V, Grigoriev M, Heslot F. Single-molecule study of RuvAB-mediated Holliday-junction migration. Proc Natl Acad Sci U S A 101, 11611-6 (2004). WholeCell: PUB_0532, PubMed: 15292508

  2. Dennis C, Fedorov A, Käs E, Salomé L, Grigoriev M. RuvAB-directed branch migration of individual Holliday junctions is impeded by sequence heterology. EMBO J 23, 2413-22 (2004). WholeCell: PUB_0534, PubMed: 15167893

  3. Grigoriev M, Hsieh P. Migration of a Holliday junction through a nucleosome directed by the E. coli RuvAB motor protein. Mol Cell 2, 373-81 (1998). WholeCell: PUB_0535, PubMed: 9774975

  4. Hiom K, West SC. Branch migration during homologous recombination: assembly of a RuvAB-Holliday junction complex in vitro. Cell 80, 787-93 (1995). WholeCell: PUB_0716, PubMed: 7889572

  5. Karymov MA, Chinnaraj M, Bogdanov A, Srinivasan AR, Zheng G, Olson WK, Lyubchenko YL. Structure, dynamics, and branch migration of a DNA Holliday junction: a single-molecule fluorescence and modeling study. Biophys J 95, 4372-83 (2008). WholeCell: PUB_0538, PubMed: 18658216

  6. ... 5 more

  7. Kowalczykowski SC, Dixon DA, Eggleston AK, Lauder SD, Rehrauer WM. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev 58, 401-65 (1994). WholeCell: PUB_0536, PubMed: 7968921

  8. Panyutin IG, Hsieh P. The kinetics of spontaneous DNA branch migration. Proc Natl Acad Sci U S A 91, 2021-5 (1994). WholeCell: PUB_0531, PubMed: 8134343

  9. Sharples GJ, Ingleston SM, Lloyd RG. Holliday junction processing in bacteria: insights from the evolutionary conservation of RuvABC, RecG, and RusA. J Bacteriol 181, 5543-50 (1999). WholeCell: PUB_0083, PubMed: 10482492

  10. Tsaneva IR, Müller B, West SC. ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli. Cell 69, 1171-80 (1992). WholeCell: PUB_0533, PubMed: 1617728

  11. West SC. Processing of recombination intermediates by the RuvABC proteins. Annu Rev Genet 31, 213-44 (1997). WholeCell: PUB_0537, PubMed: 9442895

Created 2012-10-01 15:07:41
Last updated 2012-10-01 15:14:14