MG_358 – Holliday junction DNA helicase

Name
WID	MG_358
Name	Holliday junction DNA helicase
Symbol	ruvA
Protein product	MG_358_MONOMER
Cross references	GenBank: AAC71583.1, CMR: MG_358, BioCyc: MG_358, SwissProt: Q49424
Homologs	E. coli: b1861 B. subtilis: BSU27740 M. hyopneumoniae: mhp421 M. mobile: mhp421 M. pneumoniae: MPN_535

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 456755 (nt), Length: 618 (nt), Direction: Forward, G/C content: 27.0%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 ATGATTACATCTATCTTTGGAAAAGTTACTTTTGTAGGCAAAAGAAAAAT AATTGTTGAGCACAACTGGATTTCATATTGATTTAATACAAAAGAAAACC ATAAATTTGAAAAAAATTTGGAAAAAAATAAGCAAATTTTTTGTCATATT ATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAA TACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTA TTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATT AAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGG TGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAA AATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTAT GATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAAT TCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTC AGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATT ACAAATAAAACCGCCTAA 50 100 150 200 250 300 350 400 450 500 550 600 618
Transcription unit	TU_261
Empirical formula (pH 7.5)	H₆₆₀₇C₅₉₈₈N₂₅₁₄O₄₂₆₀P₆₁₈
Molecular weight (pH 7.5; Da)	201091.36

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.4 (min) (Show evidence) Value: 5.4 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	6534700
pI	3.80

Comments
Comments	holliday junction migration
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:26
Last updated	2012-10-01 15:13:32