MG_353_DIMER – DNA-binding protein HU, putative

Name
WID	MG_353_DIMER
Name	DNA-binding protein HU, putative

Structure
Biosynthesis	[c]: (2) MG_353_MONOMER ⇒ MG_353_DIMER
No. subunits	2
DNA footprint	Length: 30 (nt), Binding: dsDNA, Region: dsDNA
Empirical formula (pH 7.5)	H₁₉₁₂C₁₁₂₆N₃₁₈O₃₁₆S₆
Molecular weight (pH 7.5; Da)	25153.48
Extinction coefficient (260 nm, 25C, pH 7.0)	-23170.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Complex subunit	DNA-binding protein HU, putative [c]: (2) MG_353_MONOMER ⇒ MG_353_DIMER

Comments
Comments	Resembles eukaryotic histones [PUB_0085]. Binds circular DNA with little sequence specificity and mass ratio 1 -- 14 beads per DNA molecule with diameter 180 ± 23 Å [PUB_0085]. 8-10 dimers have a footprint of 275 nt [PUB_0085]. Condenses DNA by factor of 2.4 [PUB_0085]. Also introduces negative supercoils inthe presence of nicking-closing enzmes [PUB_0085]. Composition Forms homodimer [PUB_0085, PUB_0377, PUB_0378].
References	Fujiwara S, Yonezawa Y. The structural information on the DNA-binding protein HU in solution obtained by the small-angle neutron scattering experiments. J Phys Chem Solids 60, 1393-5 (1999). WholeCell: PUB_0377, URL: http://www.sciencedirect.com/science/article/pii/S0022369799001262?_alid=1836137335&_rdoc=1&_fmt=high&_origin=search&_docanchor=&_ct=1&_zone=rslt_list_item&md5=58f2bf776bfab73a18a6c01e267a04a4 Rouvière-Yaniv J, Kjeldgaard NO. Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett 106, 297-300 (1979). WholeCell: PUB_0378, PubMed: 227733 Rouvière-Yaniv J, Yaniv M, Germond JE. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA. Cell 17, 265-74 (1979). WholeCell: PUB_0085, PubMed: 222478

Metadata
Created	2012-10-01 15:07:40
Last updated	2012-10-01 15:14:13