MG_353_DIMER – DNA-binding protein HU, putative

WID MG_353_DIMER View in model
Name DNA-binding protein HU, putative View in model
Biosynthesis [c]: (2) MG_353_MONOMERMG_353_DIMER View in model
No. subunits 2
DNA footprint Length: 30 (nt), Binding: dsDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H1912C1126N318O316S6
Molecular weight (pH 7.5; Da) 25153.48 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)
1200.00 View in model
Formation process Process_MacromolecularComplexation View in model
Localization c
Complex subunit DNA-binding protein HU, putative
[c]: (2) MG_353_MONOMERMG_353_DIMER
View in model
Comments Resembles eukaryotic histones [PUB_0085]. Binds circular DNA with little sequence specificity and mass ratio 1 -- 14 beads per DNA molecule with diameter 180 ± 23 Å [PUB_0085]. 8-10 dimers have a footprint of 275 nt [PUB_0085]. Condenses DNA by factor of 2.4 [PUB_0085]. Also introduces negative supercoils inthe presence of nicking-closing enzmes [PUB_0085]. Composition Forms homodimer [PUB_0085, PUB_0377, PUB_0378].
  1. Fujiwara S, Yonezawa Y. The structural information on the DNA-binding protein HU in solution obtained by the small-angle neutron scattering experiments. J Phys Chem Solids 60, 1393-5 (1999). WholeCell: PUB_0377, URL:

  2. Rouvière-Yaniv J, Kjeldgaard NO. Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett 106, 297-300 (1979). WholeCell: PUB_0378, PubMed: 227733

  3. Rouvière-Yaniv J, Yaniv M, Germond JE. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA. Cell 17, 265-74 (1979). WholeCell: PUB_0085, PubMed: 222478

Created 2012-10-01 15:07:40
Last updated 2012-10-01 15:14:13