MG_353 – DNA-binding protein HU, putative

Name
WID MG_353 View in model
Name DNA-binding protein HU, putative View in model
Protein product MG_353_MONOMER
Cross references GenBank: AAC71578.1, CMR: MG_353, BioCyc: MG_353, SwissProt: P47595
Homologs
 
Classification
Type mRNA View in model
 
Structure
Structure 448219453549 MG_350 MG_351 MG_352 MG_353 MG_354 MG_355 MG_521 View in model
Sequence Chromosome: Mgenitalium_Chr_1, Coordinate: 450719 (nt), Length: 330 (nt), Direction: Reverse, G/C content: 32.1%, Sequence:
1
51
101
151
201
251
301
ATGGAAAAAACATCAAATACAAGTAAGCCACTTTCTCGTAGTGAAATCAA
TAAAATAATTGCAGTTGCTACTGGTATAAAAGAAAAAAAAATTAAGGAAA
TCTTTAAATACCTTAACACATTGTTACTAAATGAATTGGTAAGTAGAAGT
GTTTGTATATTACCTGAAAATTTAGGTAAATTAAGGATTACTATTAGAAA
TGCACGTTATCAGAAGGATATGCAAACAGGTGAGATTAGACATATCCCAC
CAAAACCATTAGTGCGCTACAGTCCAAGCAAAACGATCAAAGAAACTGCA
GCTAAAGTGCGTTGAAAGTACGCAGACTAA
50
100
150
200
250
300
330
 View in model
Transcription unit TU_256 View in model
Empirical formula (pH 7.5) H3547C3216N1398O2255P330
Molecular weight (pH 7.5; Da) 108082.80 View in model
 
Functional genomics
Is essential
Yes (Show evidence)
 View in model
Relative expression
0.560583 (dimensionless) (Show evidence)
 View in model
Half life
4.425 (min) (Show evidence)
 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)		 3459100
pI 3.56
 
Comments
Comments Introduction Weakly homologous to DNA-binding protein HU. Canonical HU HU binds circular DNA non-specifically with a Kd of 200–2500 nM and bends DNA, possibly compacting DNA and regulating gene expression [PUB_0085, PUB_0086]. HU forms a homodimer [PUB_0085]. 8-10 HU dimers bind per 275 bp DNA [PUB_0085]. HU condenses DNA 2.4 × [PUB_0085]. HU has also been implicated in recombination and may preferentially bind holiday junctions. HU constrains negative supercoils [PUB_0086], introduces negative supercoils in the presence of nicking-closing enzyme [PUB_0085], and may preferentially binds negatively supercoiled DNA [PUB_0086]. May be regions of chromosome bound by little HU, may be concentrated around periphery of nucleoid [PUB_0087].
References

  1. Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387

  2. Dame RT, Goosen N. HU: promoting or counteracting DNA compaction?. FEBS Lett 529, 151-6 (2002). WholeCell: PUB_0086, PubMed: 12372591

  3. Drlica K, Rouviere-Yaniv J. Histonelike proteins of bacteria. Microbiol Rev 51, 301-19 (1987). WholeCell: PUB_0087, PubMed: 3118156

  4. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165

  5. Rouvière-Yaniv J, Yaniv M, Germond JE. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA. Cell 17, 265-74 (1979). WholeCell: PUB_0085, PubMed: 222478

  6. Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
 
Metadata
Created 2012-10-01 15:07:25
Last updated 2012-10-01 15:13:31