MG_352_DIMER – Holliday junction endonuclease

Name
WID	MG_352_DIMER
Name	Holliday junction endonuclease

Structure
Biosynthesis	[c]: (2) MG_352_MONOMER ⇒ MG_352_DIMER
No. subunits	2
DNA footprint	Length: 28 (nt), Binding: dsDNA, Region: dsDNA
Empirical formula (pH 7.5)	H₂₈₁₂C₁₇₈₀N₄₇₀O₄₈₈S₆
Molecular weight (pH 7.5; Da)	38796.51
Extinction coefficient (260 nm, 25C, pH 7.0)	-31860.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Enzyme	DNA recombination Holliday junction resolution [c]: (2) dRibose5P_dRibose5P + (2) H2O ⇒ (4) DR5P + (2) H
Reaction participant	DNA bound protein release (Holliday junction endonuclease releases DNA topoisomerase I) MG_122_MONOMER[d] + MG_352_DIMER[c] ⇒ MG_122_MONOMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 2mer) MG_352_DIMER[c] + MG_469_2MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_2MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases Spx subfamily protein) MG_127_MONOMER[d] + MG_352_DIMER[c] ⇒ MG_127_MONOMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DNA gyrase) DNA_GYRASE[d] + MG_352_DIMER[c] ⇒ DNA_GYRASE[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 7mer) MG_352_DIMER[c] + MG_469_7MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_7MER_ATP[c] ... 30 more DNA bound protein release (Holliday junction endonuclease releases Uncharacterized HTH-type transcriptional regulator) MG_101_MONOMER[d] + MG_352_DIMER[c] ⇒ MG_101_MONOMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases putative DNA integrity scanning protein) MG_105_OCTAMER[d] + MG_352_DIMER[c] ⇒ MG_105_OCTAMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases topoisomerase IV) MG_203_204_TETRAMER[d] + MG_352_DIMER[c] ⇒ MG_203_204_TETRAMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 4mer) MG_352_DIMER[c] + MG_469_4MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_4MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP) MG_352_DIMER[c] + MG_469_1MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_1MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases ferric uptake repressor) MG_236_MONOMER[d] + MG_352_DIMER[c] ⇒ MG_236_MONOMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA 4mer-(3)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_4MER_3ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_4MER_3ATP_ADP[c] DNA bound protein release (Holliday junction endonuclease releases adenine-specific DNA modification methylase) MG_184_DIMER[d] + MG_352_DIMER[c] ⇒ MG_184_DIMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_352_DIMER[c] ⇒ MG_094_HEXAMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 6mer) MG_352_DIMER[c] + MG_469_6MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_6MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_352_DIMER[c] ⇒ MG_205_DIMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases LuxR bacterial regulatory protein, putative) MG_352_DIMER[c] + MG_428_DIMER[d] ⇒ MG_352_DIMER[d] + MG_428_DIMER[c] DNA bound protein release (Holliday junction endonuclease releases DNA polymerase III, beta clamp) MG_001_DIMER[d] + MG_352_DIMER[c] ⇒ MG_001_DIMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA 5mer-(4)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_5MER_4ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_5MER_4ATP_ADP[c] DNA bound protein release (Holliday junction endonuclease releases single-strand binding protein family, octamer) MG_091_OCTAMER[d] + MG_352_DIMER[c] ⇒ MG_091_OCTAMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA 6mer-(5)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_6MER_5ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_6MER_5ATP_ADP[c] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 3mer) MG_352_DIMER[c] + MG_469_3MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_3MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases DNA-directed RNA polymerase) MG_352_DIMER[c] + RNA_POLYMERASE[d] ⇒ MG_352_DIMER[d] + RNA_POLYMERASE[c] DNA bound protein release (Holliday junction endonuclease releases DnaA 3mer-(2)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_3MER_2ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_3MER_2ATP_ADP[c] DNA bound protein release (Holliday junction endonuclease releases DnaA-ADP) MG_352_DIMER[c] + MG_469_1MER_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_1MER_ADP[c] DNA bound protein release (Holliday junction endonuclease releases type I restriction modification DNA specificity domain protein) MG_352_DIMER[c] + MG_438_MONOMER[d] ⇒ MG_352_DIMER[d] + MG_438_MONOMER[c] DNA bound protein release (Holliday junction endonuclease releases DNA-directed RNA polymerase holoenzyme) MG_352_DIMER[c] + RNA_POLYMERASE_HOLOENZYME[d] ⇒ MG_352_DIMER[d] + RNA_POLYMERASE_HOLOENZYME[c] DNA bound protein release (Holliday junction endonuclease releases DNA polymerase core, β-clamp, and γ-complex) DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[d] + MG_352_DIMER[c] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DNA polymerase core, β-clamp, and primase) DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[d] + MG_352_DIMER[c] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases Chromosome Segregation Protein SMC with SCP Proteins-ADP) MG_213_214_298_6MER_ADP[d] + MG_352_DIMER[c] ⇒ MG_213_214_298_6MER_ADP[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DNA ligase, NAD-dependent) MG_254_MONOMER[d] + MG_352_DIMER[c] ⇒ MG_254_MONOMER[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA-ATP 5mer) MG_352_DIMER[c] + MG_469_5MER_ATP[d] ⇒ MG_352_DIMER[d] + MG_469_5MER_ATP[c] DNA bound protein release (Holliday junction endonuclease releases DNA polymerase (2) core, β-clamp, γ-complex, and primase) DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[d] + MG_352_DIMER[c] ⇒ DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[c] + MG_352_DIMER[d] DNA bound protein release (Holliday junction endonuclease releases DnaA 2mer-(1)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_2MER_1ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_2MER_1ATP_ADP[c] DNA bound protein release (Holliday junction endonuclease releases DnaA 7mer-(6)ATP-(1)ADP) MG_352_DIMER[c] + MG_469_7MER_6ATP_ADP[d] ⇒ MG_352_DIMER[d] + MG_469_7MER_6ATP_ADP[c]
Complex subunit	Holliday junction endonuclease [c]: (2) MG_352_MONOMER ⇒ MG_352_DIMER

Parameters
Parameters	Parameter_0051: HR_RecU_CleavagePosition = 3 dimensionless Parameter_0052: HR_RecU_CleavageSequence = WTTS dimensionless

Comments
Comments	DNA-binding Sluijter et al showed that MG_352 has RecU-like resolvase activity for holliday junctions [PUB_0797]. RecU cleavage sequence WTTS is 4 bp [PUB_0538]. Makes two simultaneous cuts in DNA backbone [PUB_0079]. T4 RecU homolog endonuclease VII has alternating 10 and 14 bp arms [PUB_0740]. Composition [PUB_0079].
References	Biertümpfel C, Yang W, Suck D. Crystal structure of T4 endonuclease VII resolving a Holliday junction. Nature 449, 616-20 (2007). WholeCell: PUB_0740, PubMed: 17873859 Karymov MA, Chinnaraj M, Bogdanov A, Srinivasan AR, Zheng G, Olson WK, Lyubchenko YL. Structure, dynamics, and branch migration of a DNA Holliday junction: a single-molecule fluorescence and modeling study. Biophys J 95, 4372-83 (2008). WholeCell: PUB_0538, PubMed: 18658216 McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P, Rafferty J. The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage. Structure 13, 1341-51 (2005). WholeCell: PUB_0079, PubMed: 16154091 Sluijter M, Kaptein E, Spuesens EB, Hoogenboezem T, Hartwig NG, Van Rossum AM, Vink C. The Mycoplasma genitalium MG352-encoded protein is a Holliday junction resolvase that has a non-functional orthologue in Mycoplasma pneumoniae. Mol Microbiol 77, 1261-77 (2010). WholeCell: PUB_0797, PubMed: 20735784

Metadata
Created	2012-10-01 15:07:40
Last updated	2012-10-01 15:14:13