MG_346 – 23S rRNA methyltransferase; U2552

Name
WID	MG_346
Name	23S rRNA methyltransferase; U2552
Symbol	yibK
Protein product	MG_346_MONOMER
Cross references	GenBank: AAC71571.1, CMR: MG_346, BioCyc: MG_346, SwissProt: P47588
Homologs	E. coli: b3606 B. subtilis: BSU08930 M. hyopneumoniae: mhp636 M. mobile: mhp636 M. pneumoniae: MPN_521 S. oneidensis: SO_4529

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 443922 (nt), Length: 501 (nt), Direction: Forward, G/C content: 28.7%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 ATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAA TAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTAC ACTTAATTAAACCTTATGGCTTTTTCTTAAATGATAAAAGGATGGTTAGA GCTGGTTTAAATTGTTGAGATAAAATTCAATTATTTGAACACAAATCATG AGAACATTTCTTACAAGCAACCACTGAAAATAAAACTATTTGGCTTTTAA CTAAAAGTGGTGATAAAACTCCTGATCAAATTTGCATGACAAATAAATTA CCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAA AACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGAA ATAGTGTTAGAAGTATTAATCTTGCTAATGCAGTTGTCTGTATTTTGTAT GAATATTCAAAGCAAAATCAATACTCTAATTTAGATAAACAGTGCGCTTA A 50 100 150 200 250 300 350 400 450 500 501
Transcription unit	TU_247
Empirical formula (pH 7.5)	H₅₃₄₇C₄₈₄₅N₂₀₁₀O₃₄₈₁P₅₀₁
Molecular weight (pH 7.5; Da)	162946.34

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.5 (min) (Show evidence) Value: 5.5 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	5192700
pI	3.93

Comments
Comments	catalyzes 2'-O methyltransfer of 23S rRNA U2552 => Um [PUB_0120];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Gustafsson C, Reid R, Greene PJ, Santi DV. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes. Nucleic Acids Res 24, 3756-62 (1996). WholeCell: PUB_0120, PubMed: 8871555 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:25
Last updated	2012-10-01 15:13:30