MG_335 – GTP-binding protein engB, putative

Name
WID	MG_335
Name	GTP-binding protein engB, putative
Symbol	engB
Synonyms	yihA, ysxC
Protein product	MG_335_MONOMER
Cross references	GenBank: AAC71558.1, CMR: MG_335, BioCyc: MG_335, SwissProt: P47577
Homologs	E. coli: b3865 B. subtilis: BSU28190 M. pneumoniae: MPN_481 S. oneidensis: SO_4667

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 419470 (nt), Length: 576 (nt), Direction: Reverse, G/C content: 27.3%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 ATGGATGCACATTTTTTGAAGAGTGCAAGTGATTTAAAAGATTGTCCTCA AGATAATATCCCAGAGATTTGTTTCATGGGAAGAAGTAATGTTGGTAAAT CAAGTTTGATTAATGCTTTTTTTAAAAAGAAACTGGCTAAAACTTCAGCA ACACCAGGTCGAACACAATTACTAAATTACTTTGAATATAAAGACAAAAG ATTTGTTGATTTACCAGGTTATGGCTTTGCTAAAATAAACAAAAATAAGA AAGATTTTATTACTAATTTACTTACCCAATTTTTAAATTTTCGTAGTAAT CTAGTTGGTGTTGTTTTGATAGTAGATAGTGGAGTTGTTACTGTTCAAGA TCAGGAAGTGGTTAAAATTATTTTGCAAACAGGATTGAACTTTTTGATTG TTGCTAATAAATTTGACAAACTAAATCAAAGTGAAAGATATTTTTCGCTT AAGAATATTGCAAATTTTTTAAAAGTTAATTTTGATAAATGTGTTTTTGC AAGTACAAAAACACATCATAATCTTGCTTTAGTTCACAAAAAAATTTTCG AATTATTCGTTGAAGATGAAAGATAA 50 100 150 200 250 300 350 400 450 500 550 576
Transcription unit	TU_235
Empirical formula (pH 7.5)	H₆₁₃₁C₅₅₅₄N₂₂₆₂O₄₀₂₆P₅₇₆
Molecular weight (pH 7.5; Da)	186824.51

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	3.2 (min) (Show evidence) Value: 3.2 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	6034100
pI	4.18

Comments
Comments	protein biosynthesis [PUB_0058]; ribosome biosynthesis [PUB_0110]; B. subtilis ortholog necessary for cell division and septation [PUB_0182]; helps incorporate L16, L26, and L36 into 50S ribosome [PUB_0223, PUB_0104];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Borges CL, Parente JA, Pereira M, de Almeida Soares CM. Identification of the GTPase superfamily in Mycoplasma synoviae and Mycoplasma hyopneumoniae. Genet Mol Biol 30, 212-218 (2007). WholeCell: PUB_0058, URL: http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572007000200007&lng=en&nrm=iso&tlng=en Britton RA. Role of GTPases in bacterial ribosome assembly. Annu Rev Microbiol 63, 155-76 (2009). WholeCell: PUB_0104, PubMed: 19575570 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 ... 3 more Peterson JD, Umayam LA, Dickinson T, Hickey EK, White O. The Comprehensive Microbial Resource. Nucleic Acids Res 29, 123-5 (2001). WholeCell: PUB_0182, PubMed: 11125067, URL: http://cmr.jcvi.org/ Schaefer L, Uicker WC, Wicker-Planquart C, Foucher AE, Jault JM, Britton RA. Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. J Bacteriol 188, 8252-8 (2006). WholeCell: PUB_0223, PubMed: 16997968 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:25
Last updated	2012-10-01 15:13:28