MG_305 – chaperone

Name
WID	MG_305
Name	chaperone
Symbol	dnaK
Protein product	MG_305_MONOMER
Cross references	GenBank: AAC71527.1, CMR: MG_305, BioCyc: MG_305, SwissProt: P47547
Homologs	E. coli: b0014 B. subtilis: BSU25470 M. hyopneumoniae: mhp072 M. mobile: mhp072 M. pneumoniae: MPN_434 S. oneidensis: SO_1126

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 374919 (nt), Length: 1788 (nt), Direction: Reverse, G/C content: 36.9%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 ATGAGTGCAGACAATGGTTTAATTATTGGCATTGACCTTGGAACTACCAA TTCTTGTGTTTCTGTAATGGAAGGTGGGAGACCTGTTGTATTAGAAAATC CTGAAGGTAAAAGAACAACACCTTCCATTGTTTCCTATAAAAACAATGAA ATTATAGTAGGTGATGCTGCTAAAAGACAGATGGTTACAAACCCAAATAC CATTGTCTCCATCAAGAGGTTGATGGGTACCTCAAATAAAGTAAAAGTCC AAAATGCTGATGGTACAACTAAGGAATTAAGTCCTGAACAAGTTTCAGCG CAAATCCTTAGTTATCTTAAGGACTTTGCTGAAAAAAAGATTGGTAAAAA GATTTCAAGAGCAGTTATTACTGTTCCTGCATACTTTAATGATGCGGAAA GAAACGCTACTAAAACCGCAGGTAAGATTGCTGGTTTAAATGTTGAAAGG ATCATTAACGAACCAACTGCTGCTGCTTTAGCTTATGGGATTGATAAAGC ATCAAGAGAGATGAAAGTCTTGGTTTATGACTTGGGTGGTGGAACTTTTG ATGTATCTTTACTTGACATTGCAGAAGGTACTTTTGAAGTACTTGCAACT GCTGGGGACAACCGTTTGGGAGGTGATGATTGGGATAACAAGATCATTGA ATATATCTCAGCCTACATTGCCAAAGAACACCAGGGTTTAAACTTATCAA AAGATAAGATGGCAATGCAACGGCTTAAAGAAGCAGCTGAACGTGCTAAG ATTGAACTTTCCGCTCAACTTGAAACGATTATTTCTCTACCATTTTTAAC TGTTACCCAAAAAGGTCCTGTTAACGTTGAGTTAAAACTAACCCGTGCTA AGTTTGAGGAGTTAACAAAACCACTACTTGAAAGAACAAGAAACCCTATT TCAGATGTTATCAAGGAAGCTAAGATTAAACCTGAAGAGATTAATGAAAT TCTTTTAGTTGGTGGTTCTACAAGGATGCCTGCAGTTCAAAAGCTAGTTG AATCAATGGTACCAGGTAAAAAACCAAACCGTTCTATTAATCCTGATGAA GTTGTTGCTATTGGCGCTGCTATTCAAGGTGGGGTTTTACGTGGTGATGT TAAGGATGTTTTACTTTTAGATGTAACTCCATTAACCCTTTCTATTGAAA CTTTAGGTGGTGTGGCTACTCCTTTAATTAAGAGAAATACAACTATCCCA GTAAGTAAAAGTCAAATCTTTTCAACTGCTCAAGATAACCAAGAATCAGT TGATGTGGTTGTATGTCAAGGGGAAAGACCAATGTCTAGAGATAATAAGT CATTAGGAAGATTTAACTTAGGTGGTATTCAACCAGCACCTAAAGGTAAA CCCCAAATTGAGATTACCTTTAGTTTGGATGCCAATGGGATCTTAAATGT TAAAGCTAAGGATTTAACCACGCAAAAGGAAAACAGTATTACCATTAGTG ACAACGGTAATCTTTCTGAGGAGGAGATCCAAAAGATGATCCGTGATGCT GAAGCTAACAAGGAACGGGATAACATCATCCGTGAACGTATTGAATTACG TAATGAAGGGGAAGGTATTGTTAATACCATCAAAGAGATATTAGCAAGTC CTGATGCTAAGAATTTCCCTAAAGAAGAAAAAGAGAAGTTAGAAAAGCTA ACAGGTAACATTGATGCTGCTATTAAAGCTAATGACTATGCCAAACTCAA AGTGGAAATTGAAAACTTTAAGAAGTGAAGAGAAGAGATGGCAAAAAAAT ATAACCCAACTGGTGAACAAGGTCCACAAGCAAAATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1788
Transcription unit	TU_208
Empirical formula (pH 7.5)	H₁₉₁₇₃C₁₇₃₈₄N₇₄₅₂O₁₂₃₈₁P₁₇₈₈
Molecular weight (pH 7.5; Da)	585966.13

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.96428 (dimensionless) (Show evidence) Value: 1.96428 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.4 (min) (Show evidence) Value: 2.4 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	18455900
pI	3.79

Comments
Comments	interacts with backbone [PUB_0014]; binds short, linear, unfolded, hydrophobic peptide segments [PUB_0014]; ATP hydrolysis regulated by co-chaperone grpE [PUB_0014]; Several rounds of ATP- dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding [PUB_0096]; 1 DnaK monomer: 1 DnaJ dimer: 1 GrpE dimer [PUB_0230];
References	Eds Lund P. Molecular Chaperones in the Cell. Oxford University Press, New York (2001). WholeCell: PUB_0014, ISBN: 9780199638673 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Genevaux P, Georgopoulos C, Kelley WL. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol 66, 840-57 (2007). WholeCell: PUB_0230, PubMed: 17919282 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:23
Last updated	2012-10-01 15:13:24