MG_303 – metal ion (cobalt) ABC transporter, ATP-binding protein, putative

Name
WID	MG_303
Name	metal ion (cobalt) ABC transporter, ATP-binding protein, putative
Symbol	cbiO
Protein product	MG_303_MONOMER
Cross references	GenBank: AAC71525.1, CMR: MG_303, BioCyc: MG_303, SwissProt: P47545
Homologs	M. hyopneumoniae: mhp023 M. mobile: mhp023 M. pneumoniae: MPN_432 S. coelicolor: SCO5777

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 372926 (nt), Length: 1074 (nt), Direction: Reverse, G/C content: 29.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 ATGCAAACACCTCAAATAAAACTAGATTTAATTGAACAAAAATACCATCT TCAAGCTCAAAAAGCGAATCTTAAACTAGCAAAATTAGATTCAAAAAAAC TGTTTTCTTTTTATAAAAAGGTTGATAAAATCATTAATCCTTTTTACTTT TTAAACAGTAAAAAAAGCATTCCATTTTTTAATAGCAAATTACTGAACAT TGAACAAGATCCATTGTTTTTTAACAATATTAGTGTCTTTGTCAATAAGC ATAATGATGGTCAGATCATTAAATGTTGTTCAGGGGTAATAGAACCTAAT AAAATTACAGTTATTTTTGGTGAAAGTGGTAGTGGTAAAACTACACTAAT AAAACAGTTAGGTTTATTTGAAAAACCTAGTTTTGGCTATATAAATTGTG CTAATTATTGTTATTTTGCAAACCAATACCAACAAAAGATAACAAAAAAC TTTAAGCAAAAAATTGGTTATATTTTGCAAAAAGCAGAAGATCAGTTTTT TTGTGATTCTATTTTAGAAGAAGTTTTAACAGGAGCAGTGAACCTTAAAC TTTGTCATAAAAAAGATGTTAATTATGCCAAAAAATACCTTGATCTATGT GGATTGGAAAACATCCCGTTAATAAAAAATCCCATTGAATTAAGCGATGG ACAGAAGAAGCGTTTGGCATTAGCTAGTGTGTTAGCAATGCAAGTTAAGT TTTTAATCCTTGATGAACCTACTGTTGGGTTAGATCAAATGGCGATTAGC AACTTAAGCAAAATGCTAGTAGCAATGAAACAAACAACAAGGATTGTTAT TGTTAGCCATGATGTTGATTTTATCTTTGAAACTGCTGATAAAATCATCC ATCTCCACCAGGGAAAGATAATTCATCAAACCACTGTTAATGATTTTTTT TCAAACACCAGTTGGTTGATGCAATATGGAATCACCCCTCCAGTAATTAT CCAAGCAGTTAAGATGTTTAATGAAAAGGGAATAGCTTTTAAAAACCAAG CAGAAATTAAAAACCTTGATGATCTTATTAGTGAACTAAAAAACTTATTT TCATGCAAAAAACCAGTTTTTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1074
Transcription unit	TU_206
Empirical formula (pH 7.5)	H₁₁₄₇₀C₁₀₃₉₅N₄₃₃₅O₇₄₄₈P₁₀₇₄
Molecular weight (pH 7.5; Da)	349560.28

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	2.98349 (dimensionless) (Show evidence) Value: 2.98349 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	10.5 (min) (Show evidence) Value: 10.5 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	11202300
pI	3.89

Comments
Comments	no predicted substrate binding protein in M. genitalium; cobalt/nickel transporter [PUB_0113, PUB_0440]; associated with the cobalamin (vitamin B12) biosynthetic pathways [PUB_0110];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. KEGG for linking genomes to life and the environment. Nucleic Acids Res 36, D480-4 (2008). WholeCell: PUB_0113, PubMed: 18077471, URL: http://www.genome.jp/kegg/ Ren Q, Chen K, Paulsen IT. TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels. Nucleic Acids Res 35, D274-9 (2007). WholeCell: PUB_0440, PubMed: 17135193, URL: http://membranetransport.org/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:23
Last updated	2012-10-01 15:13:24