MG_295 – tRNA U34 sulfurtransferase

Name
WID	MG_295
Name	tRNA U34 sulfurtransferase
Symbol	mnmA
Synonyms	asuE, trmU
Protein product	MG_295_MONOMER
Cross references	GenBank: AAC71516.1, CMR: MG_295, BioCyc: MG_295, SwissProt: P47537
Homologs	E. coli: b1133 B. subtilis: BSU27500 M. hyopneumoniae: mhp433 M. mobile: mhp433 M. pneumoniae: MPN_422 S. oneidensis: SO_2633

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 362920 (nt), Length: 1104 (nt), Direction: Reverse, G/C content: 28.2%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 ATGTCAATTATTGCAAAAACAGTTTTTATAGGTTTAAGCGGTGGTGTTGA TTCTGCTGTTAGTGCTTTACTTTTAAAAAAGCAATACCAAGAAGTTATTG GTGTTTTTATGGAATGTTGGGATGAGACACTTAATAATGATTTTTATGGT CATAAGAAAATAAATAATAACAAATCAGGTTGTTCATCTTTTCAAGACTT CCAACAGGCTAAAAAAATCGCTAATTCTTTAGGAATTAAGTTAATAAAAA AAAACTTAATTGAAGCTTATTGAAACAAAGTTTTTTTACCTATGATTCAA AGTTTCAAAAAAGGGTTAACCCCAAATCCAGACATCTGGTGTAATCGTTT TATTAAGTTTGGTTTATTGCATGATTTTTGTAAGCAAATTAACCCTAATT CTCTTTTTGCAACTGGTCATTATGCCAAAATAAACATGATAGAAAATCAG CCTTTGCTTTCTATTCCTAAAGATACCAATAAAGATCAAACTTATTTTTT AGCAAATGTTAAAAAAGAACAATTTCAGAATGTTATTTTTCCTTTAGCAG ATTTAAAAAAAATAACAGTGAGAAATATTGCTAGAGAAAATAATTGAGAA GTTGCAGATAAAAAAGATTCAACTGGAATTTGTTTTATTGGTGAAAGACA TTTCAGTGATTTTTTAAAAAACTATTTACCTGTAAAAAAAGGATTAATTA AGGATTGAAAAACCAAACAAACTATTAGTGAACATGATGGTGTTTGGTTT TATACGATTGGTCAACGCAGTGGATTAAATTTAGGGGGGTTAAAACAACG TCATTTTGTTGTTGCTAAGGATATTGAAACTAATGAATTATTTGTTTCTT GTGACAAAGAAGAATTATTGAAAACAACAATTTTATTGGATCAATTTAAC TGGTTGTATACACCAAAGCAACTTCCTAGTCAAGTTCTGGTAAGAATTAG ACATGCTCAAAAACCAGAAATTGCAAAGTTGAAATTATTATCAGATAATA AACTGGAAATAACATTTAAAAATCCTGTTATAAGTGTTGCATCTGGACAG TTTGGTGTATTATATACACTTGATCAAATTTGTTTAGGAGCAGGATTAAT TTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1104
Transcription unit	TU_199
Empirical formula (pH 7.5)	H₁₁₇₆₅C₁₀₆₆₀N₄₃₈₀O₇₆₉₆P₁₁₀₄
Molecular weight (pH 7.5; Da)	358567.81

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.29325 (dimensionless) (Show evidence) Value: 1.29325 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	5.1 (min) (Show evidence) Value: 5.1 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	11563300
pI	4.07

Comments
Comments	Catalyzes 2-thiouridination (s2U34) of wobble position (34) [PUB_0045]; hypermodification increases efficiency of wobble recognition for Lys, Gln, Glu.
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Cabedo H, Macián F, Villarroya M, Escudero JC, Martínez-Vicente M, Knecht E, Armengod ME. The Escherichia coli trmE (mnmE) gene, involved in tRNA modification, codes for an evolutionarily conserved GTPase with unusual biochemical properties. EMBO J 18, 7063-76 (1999). WholeCell: PUB_0045, PubMed: 10601028 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:23
Last updated	2012-10-01 15:13:22