MG_263 – Cof-like hydrolase

Name
WID	MG_263
Name	Cof-like hydrolase
Protein product	MG_263_MONOMER
Cross references	GenBank: AAC71485.1, BioCyc: MG_263, CMR: MG_263, SwissProt: P47505
Homologs	M. pneumoniae: MPN_381 S. coelicolor: SCO4581

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 320177 (nt), Length: 876 (nt), Direction: Forward, G/C content: 31.4%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 ATGAAAAACGAAATTAAATATCTTTATTCTGACTTGGATGGTACTATTGT TAGCTGAAATCCTAAAACAGAATTTGTTTATCAAAATAAAAGTTATAAAA ATTTCCATGAAGTTAGTGATGCTACTATTAGTGCTTTTTACCGATTGCAA CAAAAGGGAATTAAGGTTGGTATTGTTACTGGTAGGGATTATTGTCGGGT GTTATGACTTGAAAAACAACTTAGAACAGGATTGCCTACCATTACTTTAG ATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTA GATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGA AGCTGCTTATAAACTTAACAGTGGTTGAATTAGTTACTTTACTAAAAACC CTTCTGTTATCTTTGAAATTGATTATGCTTTTCTTGGCTATTTCAACCCT AACACCAAACTACAAAAAAAGTTTATAGACAGTACTGAAAATTGAGATCT TAATAAACTAAAAGTTAATCAGGTTTACTTTGATATTGATACTTGCCCCT TAGCAATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAAT GCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAA AGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAG ATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAG TGAGCTAAACACAGTGTCTCACTAATCGGTAGTAATCCCAAATGCTTTGC TCTAGCAAAATACCACACTGATAGTGTTGACAATGATGGTATTGCTAACT GGATTGAAAAAAACTTGTTATGTTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876
Transcription unit	TU_174
Empirical formula (pH 7.5)	H₉₃₄₇C₈₄₇₀N₃₅₁₀O₆₁₁₂P₈₇₆
Molecular weight (pH 7.5; Da)	285236.37

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9062300
pI	4.03

Comments
Comments	HAD-like hydrolase superfamily [PUB_0096]; most HAD proteins have phosphatase activity [PUB_0108]; several HAD catalyze dehalogenase (C-halogen bond hydrolysis), phosphonatase (C-P bond hydrolysis), and ß-phosphoglucomutase (CO-P bond hydrolysis and intramolecular phosphoryl transfer) reactions [PUB_0108]; several additional functions have been annotated for HAD proteins -- lipopolysaccharide biosynthesis, degradation of 2-phosphoglycolate produced during DNA repair, biosynthesis of serine, detoxification, glycation repair to free proteins from the glycation products derived from direct nonenzymatic glycosylation [PUB_0108]; HAD phosphatases have broad and overlapping substrate spectra [PUB_0108]; none of the HAD proteins are essential in E. coli [PUB_0108];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF. Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J Biol Chem 281, 36149-61 (2006). WholeCell: PUB_0108, PubMed: 16990279 UniProt Consortium. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 37, D169-74 (2009). WholeCell: PUB_0096, PubMed: 18836194, URL: http://www.uniprot.org/uniprot/ Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:21
Last updated	2012-10-01 15:13:17


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