MG_262 – polI-like 5'-3' exonuclease, putative

Name
WID	MG_262
Name	polI-like 5'-3' exonuclease, putative
Symbol	polA
Protein product	MG_262_MONOMER
Cross references	GenBank: AAC71483.1, CMR: MG_262, BioCyc: MG_262, SwissProt: Q49406
Homologs	B. subtilis: BSU22010 M. hyopneumoniae: mhp598 M. mobile: mhp598 M. pneumoniae: MPN_379 S. coelicolor: SCO2003 S. oneidensis: SO_4669

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 318332 (nt), Length: 876 (nt), Direction: Forward, G/C content: 30.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 ATGAAAAAAGCAATCCTGATTGATGGCAATTCCTTAGCATACCGTGCTTA TTTTGCAACGTGAAAACAAGTTGAATATGCTAAGCAAAATAATTTACCTT TCAATAATGCAATAAGAACAATGTTACTAATGTGTTGGAATTTAATTAAA GCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAAC TTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTC CAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTAT TTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGAT TGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTT ACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTG TTTCTCTGTATTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAA TTTCAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATA AGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATA GGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAA CATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAA ATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAA ACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAAT CCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTT TAGTTGAAAAATTTTCAAAAATATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876
Transcription unit	TU_173
Empirical formula (pH 7.5)	H₉₃₆₂C₈₄₈₅N₃₅₅₅O₆₀₇₄P₈₇₆
Molecular weight (pH 7.5; Da)	285453.97

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.851454 (dimensionless) (Show evidence) Value: 0.851454 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9114600
pI	3.88

Comments
Comments	polI-like 5'-3' exonuclease [PUB_0072] possibly responsible for DNA resection in the initiation of homologous recombination repair. Homologous recombination is most efficient for overhang lengths of at least 8 bases [PUB_0526]. Also has weak homology to flap endonucleases involved in long-patch base excision repair.
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Carvalho FM, Fonseca MM, Batistuzzo De Medeiros S, Scortecci KC, Blaha CA, Agnez-Lima LF. DNA repair in reduced genome: the Mycoplasma model. Gene 360, 111-9 (2005). WholeCell: PUB_0072, PubMed: 16153783 Daley JM, Wilson TE. Rejoining of DNA double-strand breaks as a function of overhang length. Mol Cell Biol 25, 896-906 (2005). WholeCell: PUB_0526, PubMed: 15657419 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:21
Last updated	2012-10-01 15:13:17