MG_252 – 23S rRNA methyltransferase; G2251

Name
WID	MG_252
Name	23S rRNA methyltransferase; G2251
Symbol	rlmB
Synonyms	yjfH
Protein product	MG_252_MONOMER
Cross references	GenBank: AAC71472.1, CMR: MG_252, BioCyc: MG_252, SwissProt: P47494
Homologs	E. coli: b4180 B. subtilis: BSU00960 M. pneumoniae: MPN_355 S. coelicolor: SCO4236 S. oneidensis: SO_3934

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 300824 (nt), Length: 729 (nt), Direction: Reverse, G/C content: 31.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 ATGAAAAAACCACGCCAACAAAGTTGCTTATTTGGTGTTAAAGCCTTTGA GGAAGCGATTAATAACCAAGTTCACATTAAATTGGTAAACATTTCCATTC GTCACAAAAAACTAATACCTTTAATTGAAGCTAAAAAAATTAATTTTCAA ATCCATTCAACTAACTGATTTAACAATCAGTATAGAGATATTAACCACCA GGAATTGGTAGCAGTTTTAGATACTAACCAACTCTTAATTCCACTTGATC AACTGGTTAAAGTAGTTGAAAATAAGAAGTGCAGTACACTTGTGATGTTA GATGAGATCCAAGACCCTTATAATTTTGGTGCTATACTAAGAACTTGTTT GGCTAGTGAGGTTGATGGAATTATCTTTAAAAAGAATAACCAAGTCCCTA TCAACAATACAGTCATGAAAACTAGTATGGGTAGTGTTTTTTATCAAAAC TTAGTGCAAGTAGCTAACTTAAGTTATACGATCACTAAGCTAAAAGAAAT AGGATTTTGAACTGTTGTTTCAACTCTGGATCCTATTTGAAAACCAATTG ATTATCGCAAAGTTGATTTTGCTAAAAAAATCTTAATTGTTGGCAATGAG GATAGGGGTGTGAATCAACTAATAACAAAGAATGCAGATTGTAGAATCAA AATTCCTATGAACAATAAGATCAATTCTTTAAATGTAAGTGTTGCTTTAG GGATTATCTTGTTTGCTTGAAAAAACTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729
Transcription unit	TU_165
Empirical formula (pH 7.5)	H₇₇₉₀C₇₀₆₀N₂₉₅₅O₅₀₆₂P₇₂₉
Molecular weight (pH 7.5; Da)	237605.74

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.560583 (dimensionless) (Show evidence) Value: 0.560583 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	3.3 (min) (Show evidence) Value: 3.3 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	7571300
pI	3.90

Comments
Comments	catalyzes 2'-O methyltransfer of Gm2251 modification of 23S rRNA [PUB_0105];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Peil L. Ribosome assembly factors in Escherichia coli. Tartu University (2009). WholeCell: PUB_0105, URL: http://dspace.utlib.ee/dspace/handle/10062/10503 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:21
Last updated	2012-10-01 15:13:16