MG_248

Name
WID	MG_248
Protein product	MG_248_MONOMER
Cross references	GenBank: AAC71468.1, CMR: MG_248, BioCyc: MG_248, SwissProt: P47490
Homologs	B. subtilis: BSU25180 M. pneumoniae: MPN_351

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 295473 (nt), Length: 657 (nt), Direction: Reverse, G/C content: 27.5%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 ATGAAAAAAAGGATTAGCACAATTGCTAATTTAGTTCAATCATTTAATCC TAAGCTAGTGTATGACATCGGTTGTGATCATTCCTATCTAACATCCTATC TAATAAAAACTAATCAAAACCTAACCATTGTTAACAGTGATATTAGTAAA AATGCTTTATTAAGTAATTACCAAAAATTTAAAAATAACAATAATATCCA CTTCTTTGTTAGTGATGGATTTAATAACCTTCCAGAACTAAATATTAATC CCAAAATAGGGGTAATTGCTGGACTTGGTGGATTGAAAATCATTAACATA ATTAGCCAAAAAGAAAACTTTATTAATCGTTTTGTAATCCAACCCCAATC TAATTTAATTGAATTACGTAGTTTTTTAAGTTTAAACAGTTGGGATATTG TTAATGAAACACTAGTTCAGGATCGAGAGTTTATATATCCTATTTTGGTT ATTGAAAAACTAAAAAAACCGTTTAAATTAACAAAGGAATTGGTTATTTT AGGACCAAAGCTAATTAACTTTAAAGATAAACACTGTTTAATGAAACACT ACCAATGCCTATTGAGAGTTTATCAACCCAAACAAAAACCTAGCTTGATG GATCTTAAGATAATTGAAACATTAAATAAGATAATTACAAGCTATGAATC AAGCTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 657
Transcription unit	TU_163
Empirical formula (pH 7.5)	H₇₀₁₅C₆₃₅₇N₂₆₄₆O₄₅₅₀P₆₅₇
Molecular weight (pH 7.5; Da)	213631.22

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.369079 (dimensionless) (Show evidence) Value: 0.369079 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	6882800
pI	3.87

Comments
Comments	Rossmann-fold NAD(P)(+)-binding proteins [PUB_0110];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Chen J, Anderson JB, DeWeese-Scott C, Fedorova ND, Geer LY, He S, Hurwitz DI, Jackson JD, Jacobs AR, Lanczycki CJ, Liebert CA, Liu C, Madej T, Marchler-Bauer A, Marchler GH, Mazumder R, Nikolskaya AN, Rao BS, Panchenko AR, Shoemaker BA, Simonyan V, Song JS, Thiessen PA, Vasudevan S, Wang Y, Yamashita RA, Yin JJ, Bryant SH. MMDB: Entrez's 3D-structure database. Nucleic Acids Res 31, 474-7 (2003). WholeCell: PUB_0110, PubMed: 12520055, URL: http://www.ncbi.nlm.nih.gov/Structure/ Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:21
Last updated	2012-10-01 15:13:15