MG_244_DIMER – 3-5' helicase

Name
WID	MG_244_DIMER
Name	3-5' helicase

Structure
Biosynthesis	[c]: (2) MG_244_MONOMER ⇒ MG_244_DIMER
No. subunits	2
DNA footprint	Length: 15 (nt), Binding: dsDNA, Region: dsDNA
Empirical formula (pH 7.5)	H₁₁₇₉₄C₇₄₆₂N₁₉₂₂O₂₁₆₄S₂₈
Molecular weight (pH 7.5; Da)	163952.42
Extinction coefficient (260 nm, 25C, pH 7.0)	-154810.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Enzyme	DNA excision [c]: ATP + H2O ⇒ ADP + H + PI
Reaction participant	DNA bound protein release (3-5' helicase releases ferric uptake repressor) MG_236_MONOMER[d] + MG_244_DIMER[c] ⇒ MG_236_MONOMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DnaA 7mer-(6)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_7MER_6ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_7MER_6ATP_ADP[c] DNA bound protein release (3-5' helicase releases DNA topoisomerase I) MG_122_MONOMER[d] + MG_244_DIMER[c] ⇒ MG_122_MONOMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DnaA-ATP 3mer) MG_244_DIMER[c] + MG_469_3MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_3MER_ATP[c] DNA bound protein release (3-5' helicase releases DNA polymerase III, beta clamp) MG_001_DIMER[d] + MG_244_DIMER[c] ⇒ MG_001_DIMER[c] + MG_244_DIMER[d] ... 30 more DNA bound protein release (3-5' helicase releases DnaA-ATP 5mer) MG_244_DIMER[c] + MG_469_5MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_5MER_ATP[c] DNA bound protein release (3-5' helicase releases DnaA-ATP 2mer) MG_244_DIMER[c] + MG_469_2MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_2MER_ATP[c] DNA bound protein release (3-5' helicase releases DNA polymerase core, β-clamp, and γ-complex) DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[d] + MG_244_DIMER[c] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_GAMMA_COMPLEX[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases Chromosome Segregation Protein SMC with SCP Proteins-ADP) MG_213_214_298_6MER_ADP[d] + MG_244_DIMER[c] ⇒ MG_213_214_298_6MER_ADP[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DnaA 3mer-(2)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_3MER_2ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_3MER_2ATP_ADP[c] DNA bound protein release (3-5' helicase releases DnaA-ATP 7mer) MG_244_DIMER[c] + MG_469_7MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_7MER_ATP[c] DNA bound protein release (3-5' helicase releases DnaA-ATP 6mer) MG_244_DIMER[c] + MG_469_6MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_6MER_ATP[c] DNA bound protein release (3-5' helicase releases DNA gyrase) DNA_GYRASE[d] + MG_244_DIMER[c] ⇒ DNA_GYRASE[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases single-strand binding protein family, octamer) MG_091_OCTAMER[d] + MG_244_DIMER[c] ⇒ MG_091_OCTAMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases Spx subfamily protein) MG_127_MONOMER[d] + MG_244_DIMER[c] ⇒ MG_127_MONOMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases Uncharacterized HTH-type transcriptional regulator) MG_101_MONOMER[d] + MG_244_DIMER[c] ⇒ MG_101_MONOMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases LuxR bacterial regulatory protein, putative) MG_244_DIMER[c] + MG_428_DIMER[d] ⇒ MG_244_DIMER[d] + MG_428_DIMER[c] DNA bound protein release (3-5' helicase releases DnaA 5mer-(4)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_5MER_4ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_5MER_4ATP_ADP[c] DNA bound protein release (3-5' helicase releases DnaA 2mer-(1)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_2MER_1ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_2MER_1ATP_ADP[c] DNA bound protein release (3-5' helicase releases DnaA-ATP 4mer) MG_244_DIMER[c] + MG_469_4MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_4MER_ATP[c] DNA bound protein release (3-5' helicase releases DnaA 4mer-(3)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_4MER_3ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_4MER_3ATP_ADP[c] DNA bound protein release (3-5' helicase releases type I restriction modification DNA specificity domain protein) MG_244_DIMER[c] + MG_438_MONOMER[d] ⇒ MG_244_DIMER[d] + MG_438_MONOMER[c] DNA bound protein release (3-5' helicase releases DNA ligase, NAD-dependent) MG_244_DIMER[c] + MG_254_MONOMER[d] ⇒ MG_244_DIMER[d] + MG_254_MONOMER[c] DNA bound protein release (3-5' helicase releases DnaA 6mer-(5)ATP-(1)ADP) MG_244_DIMER[c] + MG_469_6MER_5ATP_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_6MER_5ATP_ADP[c] DNA bound protein release (3-5' helicase releases DNA polymerase core, β-clamp, and primase) DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[d] + MG_244_DIMER[c] ⇒ DNA_POLYMERASE_CORE_BETA_CLAMP_PRIMASE[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DNA polymerase (2) core, β-clamp, γ-complex, and primase) DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[d] + MG_244_DIMER[c] ⇒ DNA_POLYMERASE_2CORE_BETA_CLAMP_GAMMA_COMPLEX_PRIMASE[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases adenine-specific DNA modification methylase) MG_184_DIMER[d] + MG_244_DIMER[c] ⇒ MG_184_DIMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases topoisomerase IV) MG_203_204_TETRAMER[d] + MG_244_DIMER[c] ⇒ MG_203_204_TETRAMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases heat-inducible transcription repressor HrcA, putative) MG_205_DIMER[d] + MG_244_DIMER[c] ⇒ MG_205_DIMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DNA-directed RNA polymerase holoenzyme) MG_244_DIMER[c] + RNA_POLYMERASE_HOLOENZYME[d] ⇒ MG_244_DIMER[d] + RNA_POLYMERASE_HOLOENZYME[c] DNA bound protein release (3-5' helicase releases DnaA-ADP) MG_244_DIMER[c] + MG_469_1MER_ADP[d] ⇒ MG_244_DIMER[d] + MG_469_1MER_ADP[c] DNA bound protein release (3-5' helicase releases DNA-directed RNA polymerase) MG_244_DIMER[c] + RNA_POLYMERASE[d] ⇒ MG_244_DIMER[d] + RNA_POLYMERASE[c] DNA bound protein release (3-5' helicase releases putative DNA integrity scanning protein) MG_105_OCTAMER[d] + MG_244_DIMER[c] ⇒ MG_105_OCTAMER[c] + MG_244_DIMER[d] DNA bound protein release (3-5' helicase releases DnaA-ATP) MG_244_DIMER[c] + MG_469_1MER_ATP[d] ⇒ MG_244_DIMER[d] + MG_469_1MER_ATP[c] DNA bound protein release (3-5' helicase releases replicative DNA helicase) MG_094_HEXAMER[d] + MG_244_DIMER[c] ⇒ MG_094_HEXAMER[c] + MG_244_DIMER[d]
Complex subunit	3-5' helicase [c]: (2) MG_244_MONOMER ⇒ MG_244_DIMER

Parameters
Parameters	Parameter_0054: NER_PcrA_StepSize = 1 b

Comments
Comments	Composition [PUB_0077]. DNA-binding Step size is 1 bp [PUB_0516]. Exonuclease III footprint is 15 nt [PUB_0714]. PriA footprinti is 28 nt [PUB_0715].
References	Maluf NK, Fischer CJ, Lohman TM. A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro. J Mol Biol 325, 913-35 (2003). WholeCell: PUB_0077, PubMed: 12527299 Marians KJ. PriA: at the crossroads of DNA replication and recombination. Prog Nucleic Acid Res Mol Biol 63, 39-67 (1999). WholeCell: PUB_0715, PubMed: 10506828 Park J, Myong S, Niedziela-Majka A, Lee KS, Yu J, Lohman TM, Ha T. PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps. Cell 142, 544-55 (2010). WholeCell: PUB_0714, PubMed: 20723756 Tomko EJ, Fischer CJ, Niedziela-Majka A, Lohman TM. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell 26, 335-47 (2007). WholeCell: PUB_0516, PubMed: 17499041

Metadata
Created	2012-10-01 15:07:39
Last updated	2012-10-01 15:14:10