MG_244 – 3-5' helicase

Name
WID	MG_244
Name	3-5' helicase
Symbol	pcrA
Synonyms	recB, uvrD
Protein product	MG_244_MONOMER
Cross references	GenBank: AAC71464.1, CMR: MG_244, BioCyc: MG_244, SwissProt: P47486
Homologs	E. coli: b3813 B. subtilis: BSU06610 M. hyopneumoniae: mhp418 M. mobile: mhp418 M. pneumoniae: MPN_341 ... 2 more S. coelicolor: SCO4797 S. oneidensis: SO_0467

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 291334 (nt), Length: 2112 (nt), Direction: Forward, G/C content: 28.9%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 1801 1851 1901 1951 2001 2051 2101 ATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGT TGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCT TTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCA ATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAA ACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACA GCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATC GATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACT CGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTG CTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTA TGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAAC CCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACA ATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGT AATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTT AGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGA TGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAG ATGATTTACCGCTGAAGAGGGGCGGTAAACGGGATCATAACTGCTTTAAA GCATGACTTTAATGTTCCGAAAAGCAATGAATTCTTTATTAATCAAAATT ACCGTTGCGATCAGAATATTTTAGCAGTTGCTAACCAAATTCTTTTAAAA ATTATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTC AACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTG AAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACA GAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGA TATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTAC CTAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTA ATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGC CCTAAAAATTTGATCAAATGATTTAAAGGAATTGTGAAAACAGAGTGATA AAACAAACCTATTTGATTTTCTTAAATGATCAGAATTAAATCAAAAAAAC CATAGTTCAAAACTTAAAGCTACTGGTTATTTTAATCTGCTGATTAAGTT AGCAGAGGATCAGCAAATTAACCTTTTGTTTACTGAACTGTTTAAAAAAC TCAAAGTGGATCAAACTATTGAAAATCTGCTTTGAAAAAAACTAACTGAA TTTCAAAAAGATAAAACTGAATTTAGCTTATCAGAGTTTATTACTAGCTT AGCATTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATT TGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATT TATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAA TGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCA CAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAAC AATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTA TTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTT TTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGT TTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGG AGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACA CACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAA GCTTTATATTAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1800 1850 1900 1950 2000 2050 2100 2112
Transcription unit	TU_159
Empirical formula (pH 7.5)	H₂₂₅₅₈C₂₀₄₄₅N₈₅₃₅O₁₄₆₃₄P₂₁₁₂
Molecular weight (pH 7.5; Da)	687394.04

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	0.242995 (dimensionless) (Show evidence) Value: 0.242995 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	3.5 (min) (Show evidence) Value: 3.5 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	22135800
pI	3.88

Comments
Comments	PcrA is a 3-5' helicase that removes oligonucleotides cleaved by UvrABC during nucleotide excision repair [PUB_0072]. Oligonucleotide excision is powered by ATP hydrolysis [PUB_0531]. Several groups have studied PcrA energetics and kinetics. Wigley report that 1-23 bases are excised per ATP [PUB_0541]; Tomko et al report that PcrA catalyzes excision at the rate 193 nt/s and 1 ATP/nt [PUB_0516]. PcrA is active as a dimer [PUB_0541]. Additionally, PcrA may play a role in the initiation of recombination repair with pol I exonuclease [PUB_0072]. pcrA is also homologous to recB [PUB_0072].
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Carvalho FM, Fonseca MM, Batistuzzo De Medeiros S, Scortecci KC, Blaha CA, Agnez-Lima LF. DNA repair in reduced genome: the Mycoplasma model. Gene 360, 111-9 (2005). WholeCell: PUB_0072, PubMed: 16153783 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Panyutin IG, Hsieh P. The kinetics of spontaneous DNA branch migration. Proc Natl Acad Sci U S A 91, 2021-5 (1994). WholeCell: PUB_0531, PubMed: 8134343 Tomko EJ, Fischer CJ, Niedziela-Majka A, Lohman TM. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell 26, 335-47 (2007). WholeCell: PUB_0516, PubMed: 17499041 ... 2 more Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Wigley DB. DNA helicases: one small step for PcrA, one giant leap for RecBC?. Curr Biol 10, R444-6 (2000). WholeCell: PUB_0541, PubMed: 10873794

Metadata
Created	2012-10-01 15:07:20
Last updated	2012-10-01 15:13:14