MG_239_HEXAMER – ATP-dependent protease La

Name
WID	MG_239_HEXAMER
Name	ATP-dependent protease La
Cross references	PDB: 2ANE

Structure
Biosynthesis	[c]: (6) MG_239_MONOMER ⇒ MG_239_HEXAMER
No. subunits	6
Empirical formula (pH 7.5)	H₃₉₃₀₆C₂₄₁₂₆N₆₄₆₈O₇₂₉₆S₁₀₂
Molecular weight (pH 7.5; Da)	539984.25
Extinction coefficient (260 nm, 25C, pH 7.0)	-291750.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1200.00

Synthesis
Formation process	Process_MacromolecularComplexation
Localization	c

Function
Enzyme	Protein degradation (ALA) [c]: AlaAla + ATP + (2) H2O ⇒ ADP + (2) ALA + H + PI Protein degradation (ARG) [c]: ArgArg + ATP + (2) H2O ⇒ ADP + (2) ARG + H + PI Protein degradation (ASN) [c]: AsnAsn + ATP + (2) H2O ⇒ ADP + (2) ASN + H + PI Protein degradation (ASP) [c]: AspAsp + ATP + (2) H2O ⇒ ADP + (2) ASP + H + PI Protein degradation (CYS) [c]: ATP + CysCys + (2) H2O ⇒ ADP + (2) CYS + H + PI ... 15 more Protein degradation (GLN) [c]: ATP + GlnGln + (2) H2O ⇒ ADP + (2) GLN + H + PI Protein degradation (GLU) [c]: ATP + GluGlu + (2) H2O ⇒ ADP + (2) GLU + H + PI Protein degradation (GLY) [c]: ATP + GlyGly + (2) H2O ⇒ ADP + (2) GLY + H + PI Protein degradation (HIS) [c]: ATP + (2) H2O + HisHis ⇒ ADP + H + (2) HIS + PI Protein degradation (ILE) [c]: ATP + (2) H2O + IleIle ⇒ ADP + H + (2) ILE + PI Protein degradation (LEU) [c]: ATP + (2) H2O + LeuLeu ⇒ ADP + H + (2) LEU + PI Protein degradation (LYS) [c]: ATP + (2) H2O + LysLys ⇒ ADP + H + (2) LYS + PI Protein degradation (MET) [c]: ATP + (2) H2O + MetMet ⇒ ADP + H + (2) MET + PI Protein degradation (PHE) [c]: ATP + (2) H2O + PhePhe ⇒ ADP + H + (2) PHE + PI Protein degradation (PRO) [c]: ATP + (2) H2O + ProPro ⇒ ADP + H + PI + (2) PRO Protein degradation (SER) [c]: ATP + (2) H2O + SerSer ⇒ ADP + H + PI + (2) SER Protein degradation (THR) [c]: ATP + (2) H2O + ThrThr ⇒ ADP + H + PI + (2) THR Protein degradation (TRP) [c]: ATP + (2) H2O + TrpTrp ⇒ ADP + H + PI + (2) TRP Protein degradation (TYR) [c]: ATP + (2) H2O + TyrTyr ⇒ ADP + H + PI + (2) TYR Protein degradation (VAL) [c]: ATP + (2) H2O + ValVal ⇒ ADP + H + PI + (2) VAL
Complex subunit	ATP-dependent protease La [c]: (6) MG_239_MONOMER ⇒ MG_239_HEXAMER

Parameters
Parameters	Parameter_0097: lonProteaseEnergyCost = 6 ATP/cleavage Parameter_0098: lonProteaseFragmentLength = 20 aa Parameter_0099: lonProteaseSpecificRate = 1.667 1/s

Comments
Comments	Very processive protease; processes entire peptides without falling off. Can catalyze 10,000 cleavages per min. Degrades peptides in both directions [PUB_0029]. Cleavage site is 2-4 hydrophobic residues preceded by a charged or hydrophilic residue. Produces amino acid fragments of length 5-30 amino acids at a cost of 4 ATP due to stochastic distribution of cleavage sites [PUB_0029]. Degrades ssrA-tagged polypeptides [PUB_0821]. Also has chaperone-like activity for macromolecular complexes [PUB_0029]. Composition Function as a hexamer with Mg2+ cofactor [PUB_0029].
References	Gur E, Sauer RT. Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease. Proc Natl Acad Sci U S A 105, 16113-8 (2008). WholeCell: PUB_0821, PubMed: 18852454 Lee I, Suzuki CK. Functional mechanics of the ATP-dependent Lon protease- lessons from endogenous protein and synthetic peptide substrates. Biochim Biophys Acta 1784, 727-35 (2008). WholeCell: PUB_0029, PubMed: 18359303

Metadata
Created	2012-10-01 15:07:39
Last updated	2012-10-01 15:14:10