MG_238 – trigger factor

Name
WID	MG_238
Name	trigger factor
Symbol	tig
Protein product	MG_238_MONOMER
Cross references	GenBank: AAC71459.1, CMR: MG_238, BioCyc: MG_238, SwissProt: P47480
Homologs	E. coli: b0436 B. subtilis: BSU28230 M. hyopneumoniae: mhp233 M. mobile: mhp233 M. pneumoniae: MPN_331 ... 2 more S. coelicolor: SCO2620 S. oneidensis: SO_1793

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 281993 (nt), Length: 1335 (nt), Direction: Forward, G/C content: 32.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 ATGAAGTTATACAAAGTTCTTAACAGTAAAACAACTGATAAAAGTCTTTG TTTGGAAGTTGAGATTGATCCAAATTACTGACAAGCTACCCAAAAAAAAC TAGTAGGTGAAATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGT AAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAAT GCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAAC AAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACC ATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTT TGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATT TAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAA AAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACT AGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACA AAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCA AATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAA CCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGG AGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAA AAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCC TGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTC ATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAAC CAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCC AGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAA GCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCT AATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGA AAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGA TTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCT GTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAA TATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAA CAACAACTAAAATCGTTAGCTATTCAACTCATTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1335
Transcription unit	TU_154
Empirical formula (pH 7.5)	H₁₄₃₀₄C₁₂₉₆₈N₅₅₂₉O₉₂₀₄P₁₃₃₅
Molecular weight (pH 7.5; Da)	436223.30

Functional genomics
Is essential	No (Show evidence) Value: False Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	4.05584 (dimensionless) (Show evidence) Value: 4.05584 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	2.3 (min) (Show evidence) Value: 2.3 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	13833400
pI	3.73

Comments
Comments	Trigger factor binds translating ribosomes [PUB_0005]: Trigger factor concentration: 50 μM Ribosome concentration: 30 μM Trigger factor-50S ribosome: KD=1 μM Trigger factor-trigger factor: KD=1-2 μM Trigger factor binds ribosome 10 s Trigger factor remains bound to polypeptide for up to 35 more seconds after dissociates from ribosome
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Kaiser CM, Chang HC, Agashe VR, Lakshmipathy SK, Etchells SA, Hayer-Hartl M, Hartl FU, Barral JM. Real-time observation of trigger factor function on translating ribosomes. Nature 444, 455-60 (2006). WholeCell: PUB_0005, PubMed: 17051157 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:20
Last updated	2012-10-01 15:13:14