MG_229_231_TETRAMER_ox – oxidized ribonucleoside-diphosphate reductase

Name
WID	MG_229_231_TETRAMER_ox
Name	oxidized ribonucleoside-diphosphate reductase

Structure
Biosynthesis	[c]: MG_229_231_TETRAMER + O2 ⇒ (4) H + MG_229_231_TETRAMER_ox
No. subunits	0
Disulfide bonds (pH 7.5)	MG_231_MONOMER: 176-413
Empirical formula (pH 7.5)	H₁₆₉₃₆C₁₁₀₂₀N₂₈₂₀O₃₂₃₈S₆₆
Molecular weight (pH 7.5; Da)	242848.95
Extinction coefficient (260 nm, 25C, pH 7.0)	283530.00
Half life (OD (600 nm) = 0.3, M9 media, 36C; min)	1199.86

Synthesis
Formation process	Process_Metabolism
Localization	c

Function
Reaction participant	ribonucleoside-diphosphate reductase maintenance [c]: (2) FMNH2 + (2) MG_124_MONOMER + MG_229_231_TETRAMER_ox ⇒ (2) FMN + (2) H2O + (2) MG_124_MONOMER_ox + MG_229_231_TETRAMER ribonucleoside-diphosphate reductase (CDP) [c]: (2) CDP + MG_229_231_TETRAMER + O2 ⇒ (2) DCDP + (2) H2O + MG_229_231_TETRAMER_ox ribonucleoside-diphosphate reductase (GDP) [c]: (2) GDP + MG_229_231_TETRAMER + O2 ⇒ (2) DGDP + (2) H2O + MG_229_231_TETRAMER_ox ribonucleoside-diphosphate reductase (ADP) [c]: (2) ADP + MG_229_231_TETRAMER + O2 ⇒ (2) DADP + (2) H2O + MG_229_231_TETRAMER_ox ribonucleoside-diphosphate reductase (UDP) [c]: MG_229_231_TETRAMER + O2 + (2) UDP ⇒ (2) DUDP + (2) H2O + MG_229_231_TETRAMER_ox
Complex subunit	oxidized ribonucleoside-diphosphate reductase [c]: MG_229_231_TETRAMER + O2 ⇒ (4) H + MG_229_231_TETRAMER_ox

Comments
Comments	During catalytic cycle NrdEF complex obtains [PUB_0160] oxygen coordinated to tryosine 126 of beta subunit (NrdF, MG_229) disulfide bonds between C176 and C413 of alpha subunit (NrdE, MG_231) NrdEF is recycled by [PUB_0160] NrdI removes NrdF oxygens NrdH (or Trx in organisms such as M. genitalium which lack NrdH) reduces NrdE disulfide bonds
References	Cotruvo JA Jr, Stubbe J. NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase. Proc Natl Acad Sci U S A 105, 14383-8 (2008). WholeCell: PUB_0160, PubMed: 18799738

Metadata
Created	2012-10-01 15:07:39
Last updated	2012-10-01 15:14:10