MG_223

Name
WID	MG_223
Protein product	MG_223_MONOMER
Cross references	GenBank: AAC71444.1, CMR: MG_223, BioCyc: MG_223, SwissProt: P47465
Homologs	M. pneumoniae: MPN_316

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 268012 (nt), Length: 1236 (nt), Direction: Forward, G/C content: 31.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 ATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCA GATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTT ACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAAC AAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTA TTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGA TTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGT TATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTG TGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACC ATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGC TATGTAGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATCGGGGA GTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAGAAAATTA TTAGTCACCACTTAGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAAC ATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGA CAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTA TTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTT GATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATAC AGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAA TGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGT GGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTA TTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTG CACTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATC CAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCTTGCA GATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTG ATAATATTCAAAAACCTAAACCAAAAAAACACTTCATGATCTTATCAAAA CACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1236
Transcription unit	TU_147
Empirical formula (pH 7.5)	H₁₃₂₀₂C₁₁₉₆₅N₄₉₉₅O₈₅₉₁P₁₂₃₆
Molecular weight (pH 7.5; Da)	402712.25

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.96428 (dimensionless) (Show evidence) Value: 1.96428 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	12778200
pI	3.90

Comments
Comments	part of conserved cell division cluster (mraZ-mraW-orfX-ftsZ) [PUB_0187]; however orfX genes have low sequence similarity to each other [PUB_0187];
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Zhao Y, Hammond RW, Lee IM, Roe BA, Lin S, Davis RE. Cell division gene cluster in Spiroplasma kunkelii: functional characterization of ftsZ and the first report of ftsA in mollicutes. DNA Cell Biol 23, 127-34 (2004). WholeCell: PUB_0187, PubMed: 15000753

Metadata
Created	2012-10-01 15:07:19
Last updated	2012-10-01 15:13:11