MG_222 – S-adenosyl-methyltransferase MraW

Name
WID	MG_222
Name	S-adenosyl-methyltransferase MraW
Symbol	mraW
Protein product	MG_222_MONOMER
Cross references	GenBank: AAC71443.1, CMR: MG_222, BioCyc: MG_222, SwissProt: P47464
Homologs	E. coli: b0082 M. pneumoniae: MPN_315 S. coelicolor: SCO2092 S. oneidensis: SO_4227

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 267081 (nt), Length: 930 (nt), Direction: Forward, G/C content: 33.4%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 ATGCTAAATAACCAACAGATCCACCAGAGTGTACTGATCAATGAAGTGAT CCATAACCTCAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAG GGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACAGGAACT TTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCT TTTTTTTAAAAACAACAACGTTGTTATTATTCACGATAACTTTGCTAACT TCCCAGTTCATCTTAAACAACTATCAATAACCAAGTTTGATGGGATCTTA ATGGACCTTGGTGTATCAAGCCATCAACTCAACCAACCTAATCGCGGTTT TAGTTTTAAGAATGATGGACCGATTGACATGCGTATGGACCAATCCAATC AGAAAAATACCGCACTAACAGTTTTAAAAAACTTAACTGAACAAAAGTTA AGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGC TATTGGATTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTC AACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAA TCAAGAAACTATCTTGCCAAAGTTTTTCAAGCAATTAGGATCTATCTTAA TGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTT TAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAA AAAATTGTAAGGAATTTCATTGCAAAACTAACCAGCTTTATCCAACCTGA AGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAAC CAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGT GCCAAACTCTTTGTTATCCAAAAAAACTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930
Transcription unit	TU_147
Empirical formula (pH 7.5)	H₉₉₆₄C₉₀₃₃N₃₈₄₉O₆₄₂₆P₉₃₀
Molecular weight (pH 7.5; Da)	304064.94

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	2.98349 (dimensionless) (Show evidence) Value: 2.98349 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.0 (min) (Show evidence) Value: 4 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	9505900
pI	3.73

Comments
Comments	membrane located substrates [PUB_0126]; in E. coli in an operon that encodes genes involved in cell wall metabolism and cell division, suggesting that the target of MraW may be involved in these cellular events [PUB_0127]; lack of S-adenosylmethionine (SAM) leads to a cell division defect in E. coli, with one possible explanation being that SAM serves as a methyl donor in a required methylation event [PUB_0128]; possibly modifies FtsL and/or FtsI proteins [PUB_0128]; methylation not observed in Mycoplasma pneumoniae [PUB_0285];
References	Eds Humphery-Smith I, Hecker M. Microbial Proteomics: Functional Biology of Whole Organisms. John Wiley and Sons 49 (2006). WholeCell: PUB_0285, ISBN: 9780471699750 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Carrión M, Gómez MJ, Merchante-Schubert R, Dongarrá S, Ayala JA. mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity. Biochimie 81, 879-88 (1999). WholeCell: PUB_0126, PubMed: 10572301 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Miller DJ, Ouellette N, Evdokimova E, Savchenko A, Edwards A, Anderson WF. Crystal complexes of a predicted S-adenosylmethionine-dependent methyltransferase reveal a typical AdoMet binding domain and a substrate recognition domain. Protein Sci 12, 1432-42 (2003). WholeCell: PUB_0127, PubMed: 12824489 ... 2 more Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319 Zheng Y, Anton BP, Roberts RJ, Kasif S. Phylogenetic detection of conserved gene clusters in microbial genomes. BMC Bioinformatics 6, 243 (2005). WholeCell: PUB_0128, PubMed: 16202130

Metadata
Created	2012-10-01 15:07:19
Last updated	2012-10-01 15:13:11