MG_218 – high molecular weight cytadherence accessory protein 2

Name
WID	MG_218
Name	high molecular weight cytadherence accessory protein 2
Symbol	hmw2
Protein product	MG_218_MONOMER
Cross references	GenBank: AAC71437.1, CMR: MG_218, BioCyc: MG_218, SwissProt: P47460
Homologs	B. subtilis: BSU21350 M. pneumoniae: MPN_310 S. coelicolor: SCO5397

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 259177 (nt), Length: 5418 (nt), Direction: Forward, G/C content: 34.1%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 1551 1601 1651 1701 1751 1801 1851 1901 1951 2001 2051 2101 2151 2201 2251 2301 2351 2401 2451 2501 2551 2601 2651 2701 2751 2801 2851 2901 2951 3001 3051 3101 3151 3201 3251 3301 3351 3401 3451 3501 3551 3601 3651 3701 3751 3801 3851 3901 3951 4001 4051 4101 4151 4201 4251 4301 4351 4401 4451 4501 4551 4601 4651 4701 4751 4801 4851 4901 4951 5001 5051 5101 5151 5201 5251 5301 5351 5401 ATGAAACCATTTGATAAAAAACCTTCGCTGCAACCAATTTATGACATTGG TTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAA CCGATGTTGATAAGATCGAAAACCAGCTTTTAAAAGAGATTAAAAGCCTG GAAGATGAACTTAAAAACCTTAAGGGCTTGAAGAATCAAGCAGAAGATAA TCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTT TGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGAT AAGGTTAGTGAACTTGATAACTTAACCCGTTTTTATAAGAATGAACTAAC CCGCTTACAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATT TAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGC TTAATAGAAAATCAAAACCAAACCATTAACCGCTTAAACCAAAAGATTAA TGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCA ACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTT TACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGA GTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAA ATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATAC CAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCAC CAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATG ATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCT AAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGT TGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCC AAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAAT AAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCA AAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTG AATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCC ACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACG CAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACC AACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAA CACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAA CCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCA AAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTT AAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAA GCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAAC AACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAA CTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAA TGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAAC TAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTA TTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCG GTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATC AGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACAC AAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAA ACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTG AACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAA AAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTT AGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACG CCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAAT GAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGA GGCTAACCAAAAACAACATTCATATCAAAATCAAGCTTACTTTGAAGGTG AACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAG AAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACA TCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGT TAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGAC CAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAG TGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTA AGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGATTTATGAACTG ACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGAT TGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGG ACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTA GCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGC GCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAAC GTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAAT GAATCACTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTT CCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAA GGTTGTTTGCAAAAGAGCAAGATCAACAACGCAAAGATCAGGAGATCAAC TGACGCTTTAAACAGTTTGAAAAGGAATATACTGATTTTGATGAAGCTAA GAAAAGGGAACTTGAAGAGCTTGAAAAGATCAGAAGAAGTTTAAGTCAAA GCAACGTTGAATTAGAGAGAAAAAGAGAAAAACTGGCTACTGATTTCACT AATTTAAATAAGGTTCAACACAACACCCAAATTAACCGTGATCAACTTAA CAGTCAGATCAGACAGTTCTTATTAGAACGCAAAAACTTCCAACGCTTTA GTAATGAAGCTAATGCTAAAAAAGCCTTTTTAATTAAGCGCTTAAGAAGC TTTGCATCCAATCTAAAACTCCAAAAAGAAGCGTTAGCAATCCAAAAACT AGAGTTTGATAAGCGTGATGAACAACAGAAAAAAGAGTTACAGCAAGCTA CTTTACAACTAGAACAGTTCAAGTTTGAAAAGCAAAACTTTGACATTGAA AAACAACGCCAACTAGTTGCTATTAAAACTCAGTGTGAAAAACTTAGTGA TGAAAAAAAGGCACTAAACCAAAAGCTAGTTGAACTAAAAAACTTATCCC AAACCTATCTTGCTAATAAGAATAAGGCTGAATACTCCCAGCAACAACTC CAACAGAAATACACCAATTTACTTGATCTGAAGGAAAACTTAGAGAGAAC CAAAGATCAATTAGATAAAAAACATCGTTCTATCTTCGCTAGATTAACTA AGTTTGCAAATGACTTACGTTTTGAAAAAAAGCAACTGTTAAAAGCACAG CGCATAGTTGATGATAAAAACCGTCTTTTGAAAGAAAATGAACGTAACCT CCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATC AAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTA GCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACT GGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAAT TCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAA CTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAA AGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTA ACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATAC TTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTT AGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCA ACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTA AAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACA ACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAAT CACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAAT AAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGCGGGT GTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAG AAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATT AAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTT TCCTTACTTGTACCCCTGACTATATCCTCAACAAAAGCAAGATGATAATA CTCTGCAAATTCGTCAGCTTTTTGAACAGCAGCTGCAGTTCATGCAACAA CGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGA GAAAAAACTTGATCAAATCCAACTAGAATCCCAACTTAATAATAAGCAAA GTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACT GAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAA AGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACTCCTT CTTATCAAGACAGTGATAAACAACAGTTGTTATTTAGAATCCAAGAACTG GAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGT CCAACAACCTACTAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAA TTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAA AAAGCAGCAAGTAAATAA 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1550 1600 1650 1700 1750 1800 1850 1900 1950 2000 2050 2100 2150 2200 2250 2300 2350 2400 2450 2500 2550 2600 2650 2700 2750 2800 2850 2900 2950 3000 3050 3100 3150 3200 3250 3300 3350 3400 3450 3500 3550 3600 3650 3700 3750 3800 3850 3900 3950 4000 4050 4100 4150 4200 4250 4300 4350 4400 4450 4500 4550 4600 4650 4700 4750 4800 4850 4900 4950 5000 5050 5100 5150 5200 5250 5300 5350 5400 5418
Transcription unit	TU_143
Empirical formula (pH 7.5)	H₅₈₃₀₂C₅₂₈₈₃N₂₃₁₉₉O₃₆₉₅₃P₅₄₁₈
Molecular weight (pH 7.5; Da)	1777907.74

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	1.96428 (dimensionless) (Show evidence) Value: 1.96428 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.425 (min) (Show evidence) Value: 4.425 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	56412000
pI	3.51

Comments
Comments	required for property function of adhesins, may help localize adhesins to tip organelle, part of the cytoskeleton-like Triton shell [PUB_0088]; protein not detectable in M. genitalium [PUB_0091]; homologous to the filamentous coiled-coil portion of myosin type II heavy chain [PUB_0093]; high Pro content, low pI, central acidic Pro-rich domain [PUB_0089]; required for gliding [PUB_0091]; Pich et al showed that MG_218 codes for two proteins -- HMW2 and HMW2-s [PUB_0803]; the M. pneumoniae homolog has also been showed to code for two proteins [PUB_0804]. Pich et al also showed that MG_218 knockouts exhibit reduced gliding motility and that the MG_218 N-terminus is required for proper terminal organelle architecture [PUB_0803].
References	Balish MF, Krause DC. Mycoplasmas: a distinct cytoskeleton for wall-less bacteria. J Mol Microbiol Biotechnol 11, 244-55 (2006). WholeCell: PUB_0091, PubMed: 16983199 Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Boonmee A, Ruppert T, Herrmann R. The gene mpn310 (hmw2) from Mycoplasma pneumoniae encodes two proteins, HMW2 and HMW2-s, which differ in size but use the same reading frame. FEMS Microbiol Lett 290, 174-81 (2009). WholeCell: PUB_0804, PubMed: 19025563 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Krause DC. Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle. Trends Microbiol 6, 15-8 (1998). WholeCell: PUB_0093, PubMed: 9481818 ... 4 more Pich OQ, Burgos R, Ferrer-Navarro M, Querol E, Piñol J. Role of Mycoplasma genitalium MG218 and MG317 cytoskeletal proteins in terminal organelle organization, gliding motility and cytadherence. Microbiology 154, 3188-98 (2008). WholeCell: PUB_0803, PubMed: 18832324 Proft T, Hilbert H, Plagens H, Herrmann R. The P200 protein of Mycoplasma pneumoniae shows common features with the cytadherence-associated proteins HMW1 and HMW3. Gene 171, 79-82 (1996). WholeCell: PUB_0089, PubMed: 8675035 Razin S, Jacobs E. Mycoplasma adhesion. J Gen Microbiol 138, 407-22 (1992). WholeCell: PUB_0088, PubMed: 1593256 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:19
Last updated	2012-10-01 15:13:11