MG_216 – pyruvate kinase

Name
WID	MG_216
Name	pyruvate kinase
Symbol	pyk
Protein product	MG_216_MONOMER
Cross references	GenBank: AAC71435.1, CMR: MG_216, BioCyc: MG_216, SwissProt: P47458
Homologs	E. coli: b1676 B. subtilis: BSU29180 M. hyopneumoniae: mhp255 M. mobile: mhp255 M. pneumoniae: MPN_303 S. coelicolor: SCO5423

Classification
Type	mRNA

Structure
Structure
Sequence	Chromosome: Mgenitalium_Chr_1, Coordinate: 255595 (nt), Length: 1527 (nt), Direction: Forward, G/C content: 36.3%, Sequence: 1 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151 1201 1251 1301 1351 1401 1451 1501 ATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGC TTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATG CAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGT GGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACA ACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCC CTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAA ACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAA CACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATG CTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATC CTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACA ACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTA AAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGT GAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTA TATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAG ATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATT GAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGA TGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACC AAGTCCCTTACTGACAAAGGTACATGATTAAAGCTTGTCGCTTTTTTAAC AAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACAT CCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGG GTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCT TTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTT CTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAA ATAAAAGTAGGTTTTGACACAACGTTGTTTTACCTTTAACAAAAAAGACT GTTCCTAAAAGAAAACTTGTTAACAGTGCCTTTAAGTATGACTTTATTGT CTATCCTACTAATAACATTAACAGGATCTATGCATTATCAAACGCACGCT TAGCAGCAGCAGTTATTATTTTAACCAACAACAAACGGGTTTACACTGGC CATGGTGTTGATTATGGGATCTTCTGTTATTTAATTGATAAAAACCCCAA CCAGCTAACCAAAGCTGAACTGATTGAACTTGCTTGAAAAGCAATTAACC ACTATCAGGCTTATGGTGATTTAGAAAAACTCAAACAGTGTTTAGCTGTC TATAATGAAACAATTATCAATCTTTAG 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 1450 1500 1527
Transcription unit	TU_133
Empirical formula (pH 7.5)	H₁₆₃₅₁C₁₄₈₂₃N₆₂₉₄O₁₀₆₁₁P₁₅₂₇
Molecular weight (pH 7.5; Da)	499739.57

Functional genomics
Is essential	Yes (Show evidence) Value: True Measured: Yes Species: Mycoplasma genitalium G37, Media: SP4 + 5% CO2, Temperature (C): 37.0 References Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165
Relative expression	4.11246 (dimensionless) (Show evidence) Value: 4.11246 Measured: Yes Species: Mycoplasma pneumoniae M129, Media: Hayflick media + 20% horse serum, Temperature (C): 37.0 References Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319
Half life	4.6 (min) (Show evidence) Value: 4.6 Measured: Yes Species: Escherichia coli NCM3416, Media: M9 media + 0.2% glucose, OD600 = 0.8, Temperature (C): 30.0 References Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387
Extinction coefficient (260 nm, 25C, pH 7.0)	15660900
pI	3.87

Comments
References	Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A 99, 9697-702 (2002). WholeCell: PUB_0602, PubMed: 12119387 Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103, 425-30 (2006). WholeCell: PUB_0193, PubMed: 16407165 Weiner J 3rd, Zimmerman CU, Göhlmann HW, Herrmann R. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res 31, 6306-20 (2003). WholeCell: PUB_0569, PubMed: 14576319

Metadata
Created	2012-10-01 15:07:19
Last updated	2012-10-01 15:13:10