MG_213_214_298_6MER – chromosome segregation protein SMC

Name
WID MG_213_214_298_6MER View in model
Name chromosome segregation protein SMC View in model
 
Structure
Biosynthesis [c]: (2) MG_213_MONOMER + (2) MG_214_MONOMER + (2) MG_298_MONOMER ⇒ MG_213_214_298_6MER View in model
No. subunits 6
DNA footprint Length: 630 (nt), Binding: dsDNA, Region: dsDNA View in model
Empirical formula (pH 7.5) H27356C16998N4496O5088S76
Molecular weight (pH 7.5; Da) 378546.00 View in model
Extinction coefficient 
(260 nm, 25C, pH 7.0)		 -194330.00
Half life (OD (600 nm) = 0.3, 
M9 media, 36C; min)		 1200.00 View in model
 
Synthesis
Formation process Process_MacromolecularComplexation View in model
Localization c
 
Function
Reaction participant Chromosome condensation
[c]: ATP + H2O + MG_213_214_298_6MER ⇒ H + MG_213_214_298_6MER_ADP + PI		 View in model
Complex subunit View in model
 
Comments
Comments Composition [PUB_0336, PUB_0337]. DNA-binding Together two SMC monomers (legs) pinch loops of ~630 bp: ~90bp wind around each SMC leg, separated by a ~450 bp loop [PUB_0517,PUB_0521]. SMC complexes are spacing ~1/7130 bp [PUB_0517].
References

  1. Jensen RB, Shapiro L. Cell-cycle-regulated expression and subcellular localization of the Caulobacter crescentus SMC chromosome structural protein. J Bacteriol 185, 3068-75 (2003). WholeCell: PUB_0517, PubMed: 12730166

  2. Mascarenhas J, Volkov AV, Rinn C, Schiener J, Guckenberger R, Graumann PL. Dynamic assembly, localization and proteolysis of the Bacillus subtilis SMC complex. BMC Cell Biol 6, 28 (2005). WholeCell: PUB_0337, PubMed: 15987505

  3. Strick TR, Kawaguchi T, Hirano T. Real-time detection of single-molecule DNA compaction by condensin I. Curr Biol 14, 874-80 (2004). WholeCell: PUB_0521, PubMed: 15186743

  4. Tadesse S, Mascarenhas J, Kösters B, Hasilik A, Graumann PL. Genetic interaction of the SMC complex with topoisomerase IV in Bacillus subtilis. Microbiology 151, 3729-37 (2005). WholeCell: PUB_0336, PubMed: 16272394
 
Metadata
Created 2012-10-01 15:07:38
Last updated 2012-10-01 15:14:08